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BD Pharmingen™ PE Mouse anti-Human CD96
Clone 6F9 (RUO)
Multicolor flow cytometric analysis of CD96 expression on human peripheral blood lymphocytes. Human peripheral whole blood was stained with FITC Mouse Anti-Human CD3 (Cat. No. 555332) and with either PE Mouse IgG1 Kappa Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Human CD96 (Cat. No. 562379; Right Panel) followed by treatment with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the expression of CD3 versus CD96 (or Ig Isotype Control staining) were derived for gated events with the forward- and side-light scattering characteristics of viable lymphocytes using BD FACSDiva™ Software v. 6.1.3. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
Multicolor flow cytometric analysis of CD96 expression on human peripheral blood lymphocytes. Human peripheral whole blood was stained with FITC Mouse Anti-Human CD3 (Cat. No. 555332) and with either PE Mouse IgG1 Kappa Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Human CD96 (Cat. No. 562379; Right Panel) followed by treatment with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the expression of CD3 versus CD96 (or Ig Isotype Control staining) were derived for gated events with the forward- and side-light scattering characteristics of viable lymphocytes using BD FACSDiva™ Software v. 6.1.3. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
Multicolor flow cytometric analysis of CD96 expression on human peripheral blood lymphocytes. Human peripheral whole blood was stained with FITC Mouse Anti-Human CD3 (Cat. No. 555332) and with either PE Mouse IgG1 Kappa Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Human CD96 (Cat. No. 562379; Right Panel) followed by treatment with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the expression of CD3 versus CD96 (or Ig Isotype Control staining) were derived for gated events with the forward- and side-light scattering characteristics of viable lymphocytes using BD FACSDiva™ Software v. 6.1.3. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
Multicolor flow cytometric analysis of CD96 expression on human peripheral blood lymphocytes. Human peripheral whole blood was stained with FITC Mouse Anti-Human CD3 (Cat. No. 555332) and with either PE Mouse IgG1 Kappa Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Human CD96 (Cat. No. 562379; Right Panel) followed by treatment with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the expression of CD3 versus CD96 (or Ig Isotype Control staining) were derived for gated events with the forward- and side-light scattering characteristics of viable lymphocytes using BD FACSDiva™ Software v. 6.1.3. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
Product Details
BD Pharmingen™
TACTILE; T cell activation increased late expression
Human (QC Testing)
Mouse IgG1, κ
Human CD96 Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl
AB_2737612
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
FITC Mouse Anti-Human CD3 RUO
Size
100 Tests
Cat No.
555332
562379 Rev. 1
Antibody Details
6F9
The 6F9 monoclonal antibody specifically binds to human CD96, also known as TACTILE (T cell activation increased late expression). CD96 is a type I transmembrane glycoprotein and member of the Ig superfamily. CD96 is expressed at low levels on resting natural killer (NK) cells and T cells and at high levels on activated NK and T cells. CD96 is also expressed on some T-cell leukemia and acute myeloid leukemia cells. CD96 may serve as a marker for acute myelogenous leukemia stem cells. CD96 plays a role in the adhesive interactions of activated NK and T cells during immune responses. CD96 binds to the poliovirus receptor (CD155) that is highly expressed by some tumor cells. CD155-mediated ligation of CD96 can induce NK cell-mediated cytotoxicity. CD96-mediated uptake of CD155 may adversely affect NK cells and thus reduce their effectiveness in anti-tumor responses.
562379 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
562379 Rev.1
Citations & References
View product citations for antibody "562379" on CiteAb
Development References (4)
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Fuchs A, Cella M, Giurisato E, Shaw AS, Colonna M. Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155).. J Immunol. 2004; 172(7):3994-8. (Biology). View Reference
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Hosen N, Park CY, Tatsumi N, et al. CD96 is a leukemic stem cell-specific marker in human acute myeloid leukemia. Proc Natl Acad Sci U S A. 2007; 104(26):11008-11013. (Biology). View Reference
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Majeti R. Monoclonal antibody therapy directed against human acute myeloid leukemia stem cells. Oncogene. 2011; 30(9):1009-1019. (Biology). View Reference
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Wang PL, O'Farrell S, Clayberger C, Krensky AM. Identification and molecular cloning of tactile. A novel human T cell activation antigen that is a member of the Ig gene superfamily. J Immunol. 1992; 148(8):2600-2608. (Biology). View Reference
562379 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.