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      FITC Mouse IgG1, κ Isotype Control
      Product Details
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      BD Pharmingen™
      Mouse IgG1, κ
      Flow cytometry, Isotype control (Routinely Tested)
      20 µl
      AB_396090
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Product Notices

      1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      4. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      555748 Rev. 7
      Antibody Details
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      MOPC-21

      The MOPC-21 immunoglobulin is a mouse myeloma protein. The MOPC-21 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.

      This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only.

      555748 Rev. 7
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      555748 Rev.7
      Citations & References
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      View product citations for antibody "555748" on CiteAb

      Development References (1)

      1. Sibinovic KH, Potter M, Hoostelaere, Rode B, Wax J, ed. Catalogue of plasmacytomas and other tumors of the lymphoreticular system, 3rd edition. Kensington, Maryland: Litton Bionetics, Inc; 1976:1-33.
      555748 Rev. 7

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.