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Product Details
BD Pharmingen™
Histocompatibility-2Db; H-2Db
Mouse (QC Testing)
Mouse BALB/c IgG2b, κ
C57BL/10 mouse skin graft and splenocytes
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_394931
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
FITC Mouse IgG2b, κ Isotype Control RUO
Size
0.25 mg
Cat No.
559532
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FITC Mouse Anti-Mouse H-2D[b] RUO
Size
50 µg
Cat No.
562000
553573 Rev. 2
Antibody Details
KH95
The KH95 antibody specifically recognizes the H-2D[b] MHC class I alloantigen. Reactivity with other haplotypes (e.g., a, d, f, k, n, p, q, r, s, u, v) has not been observed.
553573 Rev. 2
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
553573 Rev.2
Citations & References
View product citations for antibody "553573" on CiteAb
Development References (1)
-
Hasenkrug KJ, Cory JM, Stimpfling JH. Monoclonal antibodies defining mouse tissue antigens encoded by the H-2 region. Immunogenetics. 1987; 25(2):136-139. (Immunogen). View Reference
553573 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.