Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
The 10-3.6 monoclonal antibody specifically recognizes the β chain of the I-A[k] MHC class II alloantigen. It cross-reacts with splenocytes from mice of the H-2[f], H-2[r], and H-2[s] haplotypes, from nonobese diabetic (NOD, H-2[g7]) mice, and with lymph node cells of BN, DA, WRA, and WRC rats. Reactivity with other haplotypes (e.g., b, d, p, q) has not been observed. Long-term in vivo treatment with 10-3.6 mAb has been reported to prevent the spontaneous development of autoimmune insulin-dependent diabetes mellitus (IDDM) in female NOD mice.
The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.