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BV750 Mouse Anti-Human IREM-2 (CD300e)
Product Details
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BD OptiBuild™
IREM2; CD300E; CD300LE; CLM2; CLM-2; CMRF35-A5; PIgR-2; LMIR6
Human (Tested in Development)
Mouse IgG2a, κ
Human IREM-2 Transfected Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
AB_2871711
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV750 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant™ Violet 750 is covered by one or more of the following US patents: 8,158,444; 8,802,450; 8,575,303; 8,455,613; 8,227,187; 8,841,072; 8,110,673.
746918 Rev. 2
Antibody Details
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UP-H1

The UP-H1 monoclonal antibody recognizes Immune receptor expressed by myeloid cells 2 (IREM-2) which is also known as CD300 antigen like family member E (CD300e), CMRF35-like molecule 2 (CLM2), or Polymeric immunoglobulin receptor 2 (PIgR2). IREM-2 is a 34 kDa type I transmembrane glycoprotein which belongs to the CMFRF35 subfamily of the immunoglobulin (Ig) superfamily. IREM-2 is expressed on monocytes, macrophages and myeloid dendritic cells (mDCs), but not on lymphoid dendritic cells or granulocytes. IREM-2 associates with DAP-12, an ITAM-containing adapter molecule, which provides activating signals. Crosslinking of IREM-2 leads to intracellular calcium mobilization, release of proinflammatory cytokines such as TNF, and increased expression of activation markers on monocytes and mDCs. IREM-2 activation of mDCs can reportedly enhance the alloreactive response of naive T cells.

The antibody was conjugated to BD Horizon™ BV750 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 750-nm. BD Horizon Brilliant BV750 can be excited by the violet laser (405 nm) and detected with a 750/30 nm filter with a 740 nm long pass. Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BV750 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended.

746918 Rev. 2
Format Details
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BV750
The BD Horizon Brilliant Violet™ 750 (BV750) dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 409-nm and an acceptor dye with an emission maximum (Em Max) at 754-nm. BV750, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 750-nm (e.g., a 750/30 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV750
Violet 405 nm
409 nm
754 nm
746918 Rev.2
Citations & References
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View product citations for antibody "746918" on CiteAb

Development References (3)

  1. Aguilar H, Alvarez-Errico D, García-Montero AC, Orfao A, Sayós J, López-Botet M. Molecular characterization of a novel immune receptor restricted to the monocytic lineage. J Immunol. 2004; 173(11):6703-6711. (Immunogen: Flow cytometry, Immunoprecipitation, Radioimmunoassay). View Reference
  2. Borrego F. The CD300 molecules: an emerging family of regulators of the immune system. Blood. 2013; 121(11):1951-1960. (Biology). View Reference
  3. Brckalo T, Calzetti F, Pérez-Cabezas B, Borràs FE, Cassatella MA, López-Botet M. Functional analysis of the CD300e receptor in human monocytes and myeloid dendritic cells.. Eur J Immunol. 2010; 40(3):722-32. (Clone-specific: Flow cytometry). View Reference
746918 Rev. 2

 

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.