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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 510 is covered by one or more of the following US patents: 8,575,303; 8,354,239.
Companion Products
The 190909 antibody specifically recognizes a common epitope on the extracellular domains of CD16 (also known as, Fc gamma RIII) and CD32 (Fc gamma RII). CD16 and CD32 serve as low affinity receptors for mouse IgG Fc constant regions and play roles in the activation or inhibition of phagocytosis, antibody-dependent cellular cytotoxicity (ADCC), degranulation, and B cell proliferation. These receptors are variably expressed on B lymphocytes, natural killer (NK) cells, monocytes, macrophages, dendritic cells, Kupffer cells, granulocytes, mast cells, immature thymocytes, and some activated mature T lymphocytes. CD16 and CD32 are single-pass type I transmembrane glycoproteins with two extracellular Ig-like domains and belong to the Ig superfamily. Ligand-bound CD16 can associate with two disulfide-linked FcR-gamma subunits (FcεRIγ) that contain cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) which deliver activating signals intracellularly. CD32 has an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and functions as an inhibitory receptor.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.
Development References (2)
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Kool M, Geurtsvankessel C, Muskens F, et al. Facilitated antigen uptake and timed exposure to TLR ligands dictate the antigen-presenting potential of plasmacytoid DCs.. J Leukoc Biol. 2011; 90(6):1177-90. (Clone-specific: Flow cytometry). View Reference
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Nimmerjahn F, Ravetch JV. FcγRs in health and disease. Curr Top Microbiol Immunol. 2011; 350:105-125. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.