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BD Horizon™ BV510 Mouse Anti-Human CD1a
Clone HI149 (RUO)
Flow cytometric analysis of CD1a expression on human MOLT-4 cells. Cells from the human MOLT-4 T lymphoblast cell line (ATCC CRL-1582) were stained with BD Horizon™ BV510 Mouse Anti-Human CD1a antibody (Cat. No. 563481/563482; solid line histogram) or with a BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of CD1a expression on human MOLT-4 cells. Cells from the human MOLT-4 T lymphoblast cell line (ATCC CRL-1582) were stained with BD Horizon™ BV510 Mouse Anti-Human CD1a antibody (Cat. No. 563481/563482; solid line histogram) or with a BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Horizon™
CD1; T6/Leu-6; R4; T6; HTA-1; FCB6
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
5 µl
V 5T CD01.01
AB_2744317
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Brilliant Violet™ 510 is a trademark of Sirigen.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
BV510 Mouse IgG1, k Isotype Control RUO
Size
50 µg
Cat No.
562946
BV510 Mouse Anti-Human CD1a RUO
Size
25 Tests
Cat No.
563482
563481 Rev. 1
Antibody Details
HI149
The HI149 monoclonal antibody specifically binds to CD1a. CD1a is a type I transmembrane glycoprotein. The 49 kDa CD1a polypeptide is associated with β2-microglobulin. CD1a is expressed on cortical thymocytes, dendritic cells and Langerhans cells. CD1a has structural similarities to the MHC class I antigen, and plays a role in antigen presentation.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.
563481 Rev. 1
Format Details
BV510
The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV510
327 nm, 405 nm
512 nm
563481 Rev.1
Citations & References
View product citations for antibody "563481" on CiteAb
Development References (4)
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Calabi F, Bradbury A. The CD1 system. Tissue Antigens. 1991; 37(1):1-9. (Biology). View Reference
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Hanau D, Schmitt DA, Bieber T, Schmitt D, Cazenave JP. Possible mechanism of action of CD1a antigens. J Invest Dermatol. 1990; 95(5):503-505. (Biology). View Reference
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
563481 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.