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Flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human Nectin-1 (CD111) antibody (Cat. No. 744894; solid line histogram) on live TF-1 cells, with corresponding Isotype Control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
BD OptiBuild™ BV421 Mouse Anti-Human Nectin-1 (CD111)
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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
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Companion Products
The CK41 monoclonal antibody specifically binds to Nectin-1, which is also known as, CD111, Poliovirus receptor-related protein 1(PRR1, PVRL1), Herpes virus entry mediator C (HveC), or Herpesvirus Ig-like receptor (HIgR). Nectin-1 is a type I transmembrane glycoprotein that belongs to the Ig gene superfamily. CD111 is expressed on a variety of cell types including, epithelial cells, endothelial cells, neuronal cells, megakaryocytes, and CD34 positive stem cells. CD111 functions as a receptor for herpes simplex virus 1 (HSV 1) and HSV 2. Nectin-1 also serves as an intercellular adhesion molecule helping to form junctions between cells, eg, between endothelial cells or epithelial cells. Nectin-1 can bind to other Nectin-1 molecules and Nectin family members and to CD155 (Poliovirus Receptor/PVR).
Development References (4)
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Krummenacher C, Baribaud I, Eisenberg RJ, Cohen GH. Cellular localization of nectin-1 and glycoprotein D during herpes simplex virus infection. J Virol. 2003; 77(16):8985-8999. (Clone-specific: Immunofluorescence). View Reference
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Krummenacher C1, Baribaud I, Ponce de Leon M, et al. Localization of a binding site for herpes simplex virus glycoprotein D on herpesvirus entry mediator C by using antireceptor monoclonal antibodies. J Virol. 2000; 74(23):10863-10872. (Immunogen: Blocking, ELISA, Flow cytometry, Immunoprecipitation, Inhibition). View Reference
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Richart SM1, Simpson SA, Krummenacher C, et al. Entry of herpes simplex virus type 1 into primary sensory neurons in vitro is mediated by Nectin-1/HveC. J Virol. 2003; 77(5):3307-3311. (Clone-specific: Blocking, Inhibition). View Reference
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Simpson SA, Manchak MD, Hager EJ, et al. Nectin-1/HveC Mediates herpes simplex virus type 1 entry into primary human sensory neurons and fibroblasts. 2005; 11(2):208-218. (Clone-specific: Blocking, Inhibition). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.