Analyses of TCL1 expression Left Panel - Two-color flow cytometric analysis of TCL1 expression in human peripheral blood lymphocytes (PBL). Human peripheral blood mononuclear cells were stained with BD Horizon™ BV421 Mouse Anti-Human CD19 antibody(Cat. No. 562440/562441). The cells were washed, fixed, and permeabilized with the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714). Cells were then stained with either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No. 565378; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human TCL1 antibody (Cat. No. 565856; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD19 versus TCL1 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Right Panel - Immunohistofluorescent staining of TCL1 in human tonsil. Following antigen retrieval with BD Pharmingen™ Retrievagen A Buffer (Cat. No. 550524), a formalin-fixed, paraffin-embedded human tonsil section was blocked using an Avidin/Biotin Blocking Kit (Vector Laboratories, Cat. No. SP-2001), then stained with Alexa Fluor® 647 Mouse Anti-Human TCL1 (Cat. No.565856 ; pseudocolored red) and BD Horizon™ BV421 Mouse Anti-Human CD45 (Cat. No. 563879/563880; pseudocolored green) antibodies. Nuclear or cytoplasmic TCL1 staining was detected in some cells. Images were acquired and merged using an epifluorescence microscopic system. Original magnification, 20×.
Analyses of TCL1 expression Left Panel - Two-color flow cytometric analysis of TCL1 expression in human peripheral blood lymphocytes (PBL). Human peripheral blood mononuclear cells were stained with BD Horizon™ BV421 Mouse Anti-Human CD19 antibody(Cat. No. 562440/562441). The cells were washed, fixed, and permeabilized with the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714). Cells were then stained with either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No. 565378; Left Plot) or Alexa Fluor® 647 Mouse Anti-Human TCL1 antibody (Cat. No. 565856; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD19 versus TCL1 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Right Panel - Immunohistofluorescent staining of TCL1 in human tonsil. Following antigen retrieval with BD Pharmingen™ Retrievagen A Buffer (Cat. No. 550524), a formalin-fixed, paraffin-embedded human tonsil section was blocked using an Avidin/Biotin Blocking Kit (Vector Laboratories, Cat. No. SP-2001), then stained with Alexa Fluor® 647 Mouse Anti-Human TCL1 (Cat. No.565856 ; pseudocolored red) and BD Horizon™ BV421 Mouse Anti-Human CD45 (Cat. No. 563879/563880; pseudocolored green) antibodies. Nuclear or cytoplasmic TCL1 staining was detected in some cells. Images were acquired and merged using an epifluorescence microscopic system. Original magnification, 20×.
Product Details
BD Pharmingen™
Oncogene TCL-1; Tcl1; Tcl1a; T-cell leukemia/lymphoma protein 1A
Human (QC Testing)
Mouse BALB/c IgG2b, κ
Human TCL1 Peptide
Intracellular staining (flow cytometry) (Routinely Tested), Immunofluorescence, Immunohistochemistry-paraffin (Tested During Development)
5 µl
AB_2739379
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Fixation/Permeabilization Kit RUO
Size
250 Tests
Cat No.
554714
Retrievagen A (pH 6.0) RUO
Cat No.
550524
Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control RUO
Size
50 µg
Cat No.
565378
BV421 Mouse Anti-Human CD19 RUO
Size
100 Tests
Cat No.
562440
565856 Rev. 1
Antibody Details
1-21
The 1-21 monoclonal antibody specifically binds to T-cell lymphoma-1 (TCL1), also known as, T-cell leukemia/lymphoma protein 1A (TCL1A). TCL1 is a ~14 kDa protein that belongs to the TCL1 protein family and is likewise known as p14TCL1. It is expressed in immature cortical thymocytes, activated T cells, pro-B cells, and a subset of naïve B cells in peripheral lymphoid tissues, but not in memory B cells and plasma cells. TCL1 plays a role in cell signaling responses. It associates with Akt at the inner plasma membrane leading to Akt phosphorylation and activation. This stimulates cell signaling responses through the MAPK/ERK pathway. TCL1 enhances T cell proliferation and survival. TCL1 is a proto-oncogene that has been associated with T-cell prolymphocytic leukemias and certain B cell neoplasias.
565856 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
565856 Rev.1
Citations & References
View product citations for antibody "565856" on CiteAb
Development References (6)
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Gaudio E, Paduano F, Ngankeu A, et al. Heat shock protein 70 regulates Tcl1 expression in leukemia and lymphomas. Blood. 2013; 121(2):351-359. (Biology). View Reference
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Herling M, Patel KA, Khalili J, et al. TCL1 shows a regulated expression pattern in chronic lymphocytic leukemia that correlates with molecular subtypes and proliferative state. Leukemia. 2006; 20(2):280-285. (Immunogen: Flow cytometry, Fluorescence microscopy, Immunofluorescence, Immunohistochemistry, Western blot). View Reference
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Herling M, Patel KA, Teitell MA, et al. High TCL1 expression and intact T-cell receptor signaling define a hyperproliferative subset of T-cell prolymphocytic leukemia. Blood. 2008; 111(1):328-337. (Immunogen: Flow cytometry, Fluorescence microscopy, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western blot). View Reference
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Palamarchuk A, Yan PS, Zanesi N, et al. Tcl1 protein functions as an inhibitor of de novo DNA methylation in B-cell chronic lymphocytic leukemia (CLL). Proc Natl Acad Sci U S A. 2012; 109(7):2555-2560. (Biology). View Reference
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Pekarsky Y, Palamarchuk A, Maximov V, et al. Tcl1 functions as a transcriptional regulator and is directly involved in the pathogenesis of CLL. Proc Natl Acad Sci U S A. 2008; 105(50):19643-19648. (Biology). View Reference
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Widhopf GF 2nd, Cui B, Ghia EM, et al. ROR1 can interact with TCL1 and enhance leukemogenesis in Eμ-TCL1 transgenic mice. Proc Natl Acad Sci U S A. 2014; 111(2):793-798. (Clone-specific: Immunoprecipitation, Western blot). View Reference
565856 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.