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BD Pharmingen™ PE Mouse Anti-Human Cytokeratin 14, 15, 16 and 19 Set
Clone KA4 (RUO)
Profile of fixed and permeabilized A431 cells with 1% paraformaldehyde and 0.1% Triton X-100 and analyzed by flow cytometry
Profile of fixed and permeabilized A431 cells with 1% paraformaldehyde and 0.1% Triton X-100 and analyzed by flow cytometry
Product Details
BD Pharmingen™
Human (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
RUO
AB_2132748
Description
Reacts with cytokeratins 14, 15, 16 and 19 present in the cytoplasm of a wide variety of human simple epithelium. Human epithelium has been shown to contain cytoplasmic filaments of the cytokeratin type. There have been 19 different polypeptides identified and they demonstrate a characteristic pattern for each type of epithelium. This reagent is useful for intracellular staining for flow cytometric analysis, western blot and immunohistochemical staining of both, acetone-fixed frozen tissue sections and formalin-fixed paraffin embedded tissue sections with citrate pretreatment.
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures
Note: This product is routinely tested on fixed and permeabilized A431 cells with 1% paraformaldehyde and 0.1% Triton X-100.
Product Notices
- This antibody has been optimized and preassayed with its matched isotype control to be used at the recommended volume of 20 ul/test. Titration of the reagents or substituting with other (non-matched) isotype control is NOT recommended.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550953 Rev. 4
Components
| Description | Quantity/Size | Part Number | Clone | Isotype |
|---|---|---|---|---|
| PE Mouse Anti-Human Cytokeratin | 100 Tests (1 ea) | 51-38255X | KA4 | IgG1, κ |
| PE Mouse IgG1 Isotype Control | 100 Tests (1 ea) | 51-35405X-6 | MOPC-21 | IgG1, κ |
550953 Rev. 4
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.