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PerCP-Cy™5.5 Mouse Anti-Human EpCAM
Product Details
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BD™
EPCAM; EGP; ESA; GA733-2; hEGP-2; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1
Human
Mouse BALB/c IgG1, λ
Breast carcinoma–associated mucin BCA-225
Flow cytometry
2 μg/mL
20 μL
4072
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

The FITC conjugate is supplied as 3 µg in 1.0 mL (3 µg/mL) of phosphate-buffered saline (PBS). The PE conjugate is supplied as 3 µg in 1.0 mL (3 µg/mL) of PBS. The PerCP-Cy5.5 conjugate is supplied as 2 µg in 1.0 mL (2 µg/mL) of PBS. The APC conjugate is supplied as 6 µg in 0.5 mL (12 µg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

347199 Rev. 1
Antibody Details
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EBA-1

Anti-EpCAM, clone EBA-1, is derived from the fusion of SP2 myeloma cells with spleen cells from BALB/c mice immunized with macromolecular aggregates containing breast carcinoma–associated mucin BCA-225.

Anti-EpCAM is directed against a human epithelial adhesion molecule (EpCAM) that has been identified as a ~40-kilodalton (kd) protein in Western blotting. This antigen is also known as epithelial surface antigen (ESA) and epithelial glycoprotein 2 (EGP-2).

347199 Rev. 1
Format Details
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PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
347199 Rev.1
Citations & References
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View product citations for antibody "347199" on CiteAb

Development References (9)

  1. Braun S, Pantel K, Müller P, et al. Cytokeratin-positive cells in the bone marrow and survival of patients with stage I, II, or III breast cancer. N Engl J Med. 2000; 342:525-533. (Biology). View Reference
  2. De Leij L, Helrich W, Stein R, Mattes MJ. SCLC-cluster-2 antibodies detect the pancarcinoma/epithelial glycoprotein EGP-2. Int J Cancer. 1994; 8:60-63. (Biology). View Reference
  3. Diel IJ, Kaufmann M, Goerner R, Costa SD, Kaul S, Bastert G. Detection of tumor cells in bone marrow of patients with primary breast cancer: a prognostic factor for distant metastasis. J Clin Oncol. 1992; 10:1534-1539. (Biology). View Reference
  4. Hardingham JE, Kotasek D, Farmer B, et al. Immunobead-PCR: a technique for the detection of circulating tumor cells using immunomagnetic beads and the polymerase chain reaction. Cancer Res. 1993; 53(15):3455-3458. (Biology). View Reference
  5. Latza U, Niedobitek G, Schwarting R, Nekarda H, Stein H. Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelial. J Clin Pathol. 1990; 43(3):213-219. (Biology). View Reference
  6. Momburg F, Moldenhauer G, Hämmerling GJ, Möller P Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues. Cancer Research. 1987; 47:2883-2891. (Biology).
  7. Naume B, Borgen E, Beiske K, et al. Immunomagnetic techniques for the enrichment and detection of isolated breast carcinoma cells in bone marrow and peripheral blood . J Hematotherapy. 1997; 103-113. (Biology).
  8. Stahel RA, Gilks WR, Lehmann HP, Schenker T. Third International Workshop on Lung Tumor and Differentiation Antigens: overview of the results of the central data analysis. Int J Cancer. 1994; 8:6-26. (Biology). View Reference
  9. Yemul S, Leon Ja, Pozniakoff T, Esser PD, Estabrook A. Radioimmunoimaging of human breast carcinoma xenografts in nude mouse model with 111In-labeled new monoclonal antibody EBA-1 and F(ab')2 fragments. Nucl Med Biol. 1993; 20:325-335. (Biology). View Reference
View All (9) View Less
347199 Rev. 1

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