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A15220_612234_image1.png

Western blot analysis of p21-Arc on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- p21-Arc antibody.

A15220_612234_image2.png

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

A15220_612234_image1.png A15220_612234_image2.png Blue-Cap-White-Label.jpg

Western blot analysis of p21-Arc on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- p21-Arc antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Chicken, Dog (Tested in Development)
Mouse IgG1
Human p21-Arc aa. 10-118
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
21 kDa
250 µg/ml
AB_399557
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
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Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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612234 Rev. 1
Antibody Details
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26/p21-Arc

Cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton.  This reorganization of actin filaments occurs through the interactions between actin and actin binding proteins.  Actin-binding proteins regulate the polymerization and depolymerization of actin, connect actin-based structures to membranes and to other cytoskeletal elements, power the movement of actin filaments, and cross-link actin filaments into bundles.  Actin related proteins (Arp) 2/3 complex is an actin polymerization inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20 Arc, and p16-Arc.  The Arp2 and Arp3 subunits may nucleate actin polymerization, while the p41-Arc subunit is a WD repeat-containing protein that may regulate both the activity and localization of the Arp2/3 complex.  Arp3, p34-Arc, and p21-Arc are localized to the lamellipodia of stationary and locomoting fibroblasts.  Both WASP and Abp1p are acidic sequence-containing proteins that activate the Arp2/3 complex.  However, WASP binds actin monomers, while the endocytosis-related Abp1p protein binds actin filaments.  Thus, Arp2/3 complex may regulate actin polymerization in specific cell locations through interaction with actin binding Arp2/3 activators.

612234 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612234 Rev.1
Citations & References
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View product citations for antibody "612234" on CiteAb

Development References (2)

  1. Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol. 1997; 138(2):375-384. (Biology). View Reference
  2. Zhao X, Yang Z, Qian M, Zhu X. Interactions among subunits of human Arp2/3 complex: p20-Arc as the hub. Biochem Biophys Res Commun. 2001; 280(2):513-517. (Biology). View Reference
612234 Rev. 1

 

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.