Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
TEF-1 (transcription enhancer factor-1) is a member of a family of transcription factors that contain an evolutionarily conserved DNA binding domain (DBD), TEA/ATTS. TEF-1 was identified as a HeLa cell transcriptional activator that bound, via its TEA domain, to the GT-IIC and Sph enhancer elements. Comparison of various enhancers indicates that TEF-1 binds to highly degenerate DNA sequences and cooperatively interacts with tandem repeats of its binding sites, which increases its enhancer activity. However, the DNA binding activities of TEF-1 are modulated by sequences outside the DBD. The N-terminal basic portion of TEF-1 contains the DBD. The C-terminal portion contains the transactivation domain and a zinc finger-like motif that is thought to be involved in protein dimerization or interaction with additional transcription factors. TEF-1 induced transcription requires a limiting transcriptional intermediary factor (TIF) and a TATA-binding protein associated factor (hTAFIIs). Although TEF-1 is expressed ubiquitously during embryogenesis, its expression is limited to adult kidney, heart, brain, skeletal muscle, and lungs. Disruption of murine TEF-1 gene results in fetal death.
Development References (5)
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Deshpande N, Chopra A, Rangarajan A, Shashidhara LS, Rodrigues V, Krishna S. The human transcription enhancer factor-1, TEF-1, can substitute for Drosophila scalloped during wingblade development. J Biol Chem. 1997; 272(16):10664-10668. (Biology). View Reference
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Gupta MP, Amin CS, Gupta M, Hay N, Zak R. Transcription enhancer factor 1 interacts with a basic helix-loop-helix zipper protein, Max, for positive regulation of cardiac alpha-myosin heavy-chain gene expression. Mol Cell Biol. 1997; 17(7):3924-3936. (Biology). View Reference
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Jacquemin P, Hwang JJ, Martial JA, Dolle P, Davidson I. A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain. J Biol Chem. 1996; 271(36):21775-21785. (Biology). View Reference
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Ueyama T, Zhu C, Valenzuela YM, Suzow JG, Stewart AF. Identification of the functional domain in the transcription factor RTEF-1 that mediates alpha 1-adrenergic signaling in hypertrophied cardiac myocytes. J Biol Chem. 2000; 275(23):17476-17480. (Clone-specific: Gel shift). View Reference
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Xiao JH, Davidson I, Matthes H, Garnier JM, Chambon P. Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1. Cell. 1991; 65(4):551-568. (Biology). View Reference
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