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Western blot analysis of Syntaxin 8 on human endothelial lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Syntaxin 8.
Immunofluorescent staining of WI38 cells.
BD Transduction Laboratories™ Purified Mouse Anti-Syntaxin 8
BD Transduction Laboratories™ Purified Mouse Anti-Syntaxin 8
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Eukaryotic protein trafficking involves the packaging of molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Recognition between vesicle and acceptor membrane is mediated by the pairing of the integral membrane SNARE proteins. The stable interaction between vesicle proteins (v-SNAREs) and target proteins (t-SNAREs) juxtaposes the membranes and results in an activated docking state and/or membrane fusion. The syntaxin protein family contains a number of members that serve as functional t-SNAREs. One member of this family, syntaxin 8, has two coiled-coil domains, one in the N-terminus and one toward the C-terminus. The C-terminal domain is homologous to a similar domain in syntaxin 6 and is predicted to interact in the formation of SNARE complexes, while a hydrophobic domain in the C-terminus may be involved in membrane anchoring. Syntaxin-8 is ubiquitously expressed, but is present at high levels in the heart and localizes specifically to the ER. Thus, syntaxin-8 is a t-SNARE that is thought to be involved in the early secretory pathway of many different cell types.
Development References (2)
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Steegmaier M, Yang B, Yoo JS. Three novel proteins of the syntaxin/SNAP-25 family. J Biol Chem. 1998; 273(51):34171-34179. (Biology). View Reference
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Thoreau V, Bergès T, Callebaut I. Molecular cloning, expression analysis, and chromosomal localization of human syntaxin 8 (STX8. Biochem Biophys Res Commun. 1999; 257(2):577-583. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.