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Western blot analysis of AIB-1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- AIB-1 antibody.
BD Transduction Laboratories™ Purified Mouse Anti- AIB-1
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
Signal transduction via nuclear hormone receptors is important for cell growth and differentiation, development, and homeostasis. Nuclear hormone receptors are ligand-activated transcription factors that modulate target gene expression. These ligand/receptor complexes also interact with transcriptional coactivators which enhance ligand-dependent transcription. Various classes of coactivators have been identified, including SRC-1 and its related proteins, such as TIF-2/GRIP-1, RIP140, AIB-1, and TIF-1α and -1β. AIB-1 (Amplified In Breast cancer-1) is also known as pCIP, RAC3, and TRAM-1. It interacts with estrogen receptor (ER) and is overexpressed in breast cancer biopsies and several breast and ovarian cancer cell lines. Similar to SRC-1 and TIF2, AIB-1 contains a basic helix-loop-helix (bHLH) domain followed by a PAS (Per/Arnt/Sim) region, serine and threonine rich regions, and a charged cluster. In addition, AIB-1 contains three copies of the conserved LXXLL motif which is critical to its interaction with the nuclear receptor. Thus, AIB-1 is a coactivator of nuclear receptors that may participate in the development of steroid-dependent cancers.
Development References (5)
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Anzick SL, Kononen J, Walker RL, et al. AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer. Science. 1997; 277(5328):965-968. (Biology). View Reference
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Eng FC, Barsalou A, Akutsu N, et al. Different classes of coactivators recognize distinct but overlapping binding sites on the estrogen receptor ligand binding domain. J Biol Chem. 1998; 273(43):28371-28377. (Biology). View Reference
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Lauritsen KJ, List HJ, Reiter R, Wellstein A, Riegel AT. A role for TGF-beta in estrogen and retinoid mediated regulation of the nuclear receptor coactivator AIB1 in MCF-7 breast cancer cells. Oncogene. 2002; 21(47):7147-7155. (Biology: Western blot). View Reference
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Louie MC, Yang HQ, Ma AH, et al. Androgen-induced recruitment of RNA polymerase II to a nuclear receptor-p160 coactivator complex. Proc Natl Acad Sci U S A. 2003; 100(5):2226-2230. (Biology: Western blot). View Reference
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Reiter R, Wellstein A, Riegel AT. An isoform of the coactivator AIB1 that increases hormone and growth factor sensitivity is overexpressed in breast cancer. J Biol Chem. 2001; 276(43):39736-39741. (Biology: Western blot). View Reference
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