ELISA Detection: Biotinylated B33-2 antibody (Cat. No. 555040) is useful as a detection antibody in sandwich ELISA for measuring human IL-2 protein levels. The biotinylated B33-2 antibody can be paired with purified MQ1-17H12 antibody (Cat. No. 554563) as the capture antibody and with recombinant human IL-2 (Cat. No. 554603) as the standard. Biotinylated B33-2 antibody should be titrated (0.5 - 2.0 µg/mL) to determine the optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of human IL-2 ranging from ~2,000 to 15 pg/mL are recommended for inclusion in each ELISA plate. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. For serum or plasma, the BD OptEIA™ Human IL-2 ELISA Set (Cat. No. 555190) or BD OptEIA™ Human IL-2 ELISA Kit II (Cat. No.550611) are recommended.
This ELISA pair has been reported to show no cross-reactivity with the following cytokines: mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL- 10, IL-12p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2 MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL- 2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).