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Purified Mouse Anti-PAI-1
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse,Rat,Dog,Chicken (Tested in Development)
Mouse IgG1
Human PAI-1 aa. 207-329
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
47 kDa
250 µg/ml
AB_399420
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612024 Rev. 1
Antibody Details
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41/PAI-1

Fibrinolysis is regulated by the plasminogen activators, tissue plasminogen activator (tPA) and urokinase PA (uPA), and by the plasminogen activator-inhibitors (PAIs). Two PAIs include the serpin family members, PAI-1 and PAI-2. PAI-1 is a glycoprotein found in plasma, platelets, endothelial cells, hepatoma cells, and fibrosarcoma cells. Thrombin, endotoxin, and IL-1 induce PAI-1 synthesis in endothelial cells, which is where the major portion of plasma PAI-1 is produced. PAI-2 is a glycoprotein expressed in placenta and monocyte macrophages. The uPA/plasmin system may play a key role in cancer progression through degradation of the extracellular matrix during tumor cell migration. Paradoxically, high levels of PAI-1 are also predictive of poor prognosis of cancer patients. This finding may be a result of the role of plasmin proteolysis in the prevention of tumor vessel assembly. PAI-1 can promote tumor angiogenesis, and the mechanism may involve PAI-induced regulation of plasmin proteolysis during tumor angiogenesis. Thus, PAI-1 is a serpin protease inhibitor that is important for the regulation of plasmin proteolysis during fibrinolysis and extracellular matrix degradation.

612024 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612024 Rev.1
Citations & References
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View product citations for antibody "612024" on CiteAb

Development References (3)

  1. Bajou K, Masson V, Gerard RD. The plasminogen activator inhibitor PAI-1 controls in vivo tumor vascularization by interaction with proteases, not vitronectin. Implications for antiangiogenic strategies. J Cell Biol. 2001; 152(4):777-784. (Biology). View Reference
  2. Cai H, Li Z, Goette A, Mera F. Downregulation of endocardial nitric oxide synthase expression and nitric oxide production in atrial fibrillation: potential mechanisms for atrial thrombosis and stroke. Circulation. 2002; 106(22):2854-2858. (Clone-specific: Western blot). View Reference
  3. Ginsburg D, Zeheb R, Yang AY. cDNA cloning of human plasminogen activator-inhibitor from endothelial cells. J Clin Invest. 1986; 78(6):1673-1680. (Biology). View Reference
612024 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.