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Western blot analysis of p150 [Glued] on a human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- p150 [Glued] antibody.
Immunoflourescent staining of human endothelial cells.
BD Transduction Laboratories™ Purified Mouse Anti-p150 [Glued]
BD Transduction Laboratories™ Purified Mouse Anti-p150 [Glued]
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
p150 [Glued] was identified as a polypeptide associated with cytoplasmic dynein, the minus-end-directed microtubule-based motor protein. p150 [Glued] is also a member of the oligomeric dynactin complex. Dynactin mediates dynein-driven vesicle motility, as well as lower eukaryote nuclear transport. p150 [Glued] bears significant homology to the product of the Glued gene in Drosophila. It has been shown in vitro to be a required activator of dynein-mediated transport along microtubules. The p150 [Glued] component of the dynactin complex binds to microtubules and the actin-like protein Centractin (Arp-1), another member of the dynactin complex. In the developing rat, p150 [Glued] is expressed at high levels in neural tissue. Microtubule bindings assays with selected constructs of p150 [Glued] indicate that amino acids 39-150 are required for microtubule association.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (5)
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Askham JM, Vaughan KT, Goodson HV, Morrison EE. Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome. Mol Biol Cell. 2002; 13(10):3627-3645. (Clone-specific: Immunofluorescence, Western blot). View Reference
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Aumais JP, Tunstead JR, McNeil RS, et al. NudC associates with Lis1 and the dynein motor at the leading pole of neurons. J Neurosci. 2001; 21(24):RC187:1-RC187:7. (Clone-specific: Immunofluorescence). View Reference
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King SJ, Schroer TA. Dynactin increases the processivity of the cytoplasmic dynein motor. Nat Cell Biol. 2000; 2(1):20-24. (Clone-specific). View Reference
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Kurzchalia T. Anthrax toxin rafts into cells. J Cell Biol. 2003; 160(3):295-296. (Clone-specific). View Reference
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Tai AW, Chuang JZ, Sung CH. Cytoplasmic dynein regulation by subunit heterogeneity and its role in apical transport. J Cell Biol. 2001; 153(7):1499-1509. (Clone-specific: Immunofluorescence, Western blot). View Reference
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