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Purified Mouse Anti-β-Catenin
Purified Mouse Anti-β-Catenin

Western blot analysis of β-Catenin on HeLa cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the Mouse Anti- β-Catenin antibody.

Purified Mouse Anti-β-Catenin

Immunofluorescent staining of A431 cell line with  the Anti- β-Catenin antibody.

Western blot analysis of β-Catenin on HeLa cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the Mouse Anti- β-Catenin antibody.

Immunofluorescent staining of A431 cell line with  the Anti- β-Catenin antibody.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Mouse β-Catenin aa. 571-781
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
92 kDa
250 µg/ml
AB_397555
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610153 Rev. 2
Antibody Details
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14/Beta-Catenin

The 14/Beta-Catenin monoclonal antibody specifically binds to Beta-Catenin  (β-Catenin). β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions (zonula adherens). Deletions in the cytoplasmic domain of E-Cadherin which eliminate catenin binding also result in a loss of cell adhesion, indicating that this binding is essential for E-Cadherin function. Although the α- and β-Catenins have been cloned, very little is known about their biochemical roles. However a link between β-Catenin and colon cancer has been described. β-Catenin was found to co-immunoprecipitate with the APC tumor suppressor protein in human colorectal tumor cell lines, as well as in human kidney 293 cells. E-Cadherin, however, was not detectable in these complexes. Thus the APC-Catenin complex may be affecting the transmission of contact inhibition signals and/or the regulation of cell adhesion.

610153 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610153 Rev.2
Citations & References
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Development References (6)

  1. Eger A, Stockinger A, Schaffhauser B, Beug H, Foisner R. Epithelial mesenchymal transition by c-Fos estrogen receptor activation involves nuclear translocation of beta-catenin and upregulation of beta-catenin/lymphoid enhancer binding factor-1 transcriptional activity. J Cell Biol. 2000; 148(1):173-187. (Clone-specific: Electron microscopy, Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  2. Fallone F, Britton S, Nieto L, Salles B, Muller C. ATR controls cellular adaptation to hypoxia through positive regulation of hypoxia-inducible factor 1 (HIF-1) expression. Oncogene. 2013; 32(37):4387-4396. (Clone-specific: Western blot). View Reference
  3. Lee MS, D'Amour KA, Papkoff J. A yeast model system for functional analysis of beta-catenin signaling. J Cell Biol. 2002; 158(6):1067-1078. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  4. Ozawa M, Ringwald M, Kemler R. Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule. Proc Natl Acad Sci U S A. 1990; 87(11):4246-4250. (Biology). View Reference
  5. Persad S, Troussard AA, McPhee TR, Mulholland DJ, Dedhar S. Tumor suppressor PTEN inhibits nuclear accumulation of beta-catenin and T cell/lymphoid enhancer factor 1-mediated transcriptional activation. J Cell Biol. 2001; 153(6):1161-1173. (Clone-specific: Gel shift, Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  6. Tateishi K, Omata M, Tanaka K, Chiba T. The NEDD8 system is essential for cell cycle progression and morphogenetic pathway in mice. J Cell Biol. 2001; 155(4):571-579. (Clone-specific: Immunofluorescence, Immunohistochemistry). View Reference
View All (6) View Less
610153 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.