BD Transduction Laboratories™ Purified Mouse Anti-Annexin II Light Chain
Clone 148/Annexin II Light Chain
(RUO)
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Annexin II often exists as a heterotetramer complexed with an 11 kDa light chain. This p11 subunit is related to the S100 family of calcium-binding proteins but mutations have rendered it unable to bind calcium. The p11 protein binds to the amino terminus of the annexin II monomer, but not to other annexins. It may regulate the function of annexin II, as the tetrameric form seems to have a higher calcium and phospholipid-binding affinity than the monomeric annexin II. In the cell, p11 has been localized to the cytoplasmic face of the plasma membrane where the annexin II tetramer is known to exist.
Development References (5)
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Choi KS, Fitzpatrick SL, Filipenko NR, et al. Regulation of plasmin-dependent fibrin clot lysis by annexin II heterotetramer. J Biol Chem. 2001; 276(27):25212-25221. (Clone-specific: Immunohistochemistry). View Reference
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Harder T, Kellner R, Parton RG, Gruenberg J. Specific release of membrane-bound annexin II and cortical cytoskeletal elements by sequestration of membrane cholesterol. Mol Cell Biol. 1997; 8(3):533-544. (Biology). View Reference
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Huang XL, Pawliczak R, Cowan MJ. Epidermal growth factor induces p11 gene and protein expression and down-regulates calcium ionophore-induced arachidonic acid release in human epithelial cells. J Biol Chem. 2002; 277(41):38431-38440. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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Kwon M, Caplan JF, Filipenko NR, et al. Identification of annexin II heterotetramer as a plasmin reductase. J Biol Chem. 2002; 277(13):10903-10911. (Clone-specific: Flow cytometry). View Reference
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Regnouf F, Sagot I, Delouche B, et al. "In vitro" phosphorylation of annexin 2 heterotetramer by protein kinase C. Comparative properties of the unphosphorylated and phosphorylated annexin 2 on the aggregation and fusion of chromaffin granule membranes. J Biol Chem. 1995; 270(45):27143-27150. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.