ELISA Capture:Biotin Mouse Anti-Human CD154 antibody (Cat. No. 552560) is useful as a detection antibody for a sandwich ELISA for measuring human CD154 (CD40L) protein levels.Biotin Mouse Anti-Human CD154 antibody should be titrated between 0.5 - 2 µg/ml to determine its optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of recombinant human soluble CD154 (CD40L), ranging from 2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology see the protocols under "ELISA and ELISPOT" at our website: http://www.bdbiosciences.com/en-us/support/bd-biosciences-resource-library.
Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: CD40, IL-1Ra, IL-1R1, IL-1RII, IL-1α, IL-1β, IL-2, IL-3, IL-4, sIL-4R, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, TNF-α, TNF-β, sTNFRI, sTNFRII, IFNγ, TRAIL, GM-CSF, TGFβ, Trx