ELISA Capture (Human/Viral IL-10): Biotinylated- JES3-12G8 (Cat. No. 554599) is useful as an ELISA capture antibody when used in combination with purified JES3-9D7 (Cat No. 554497) and with recombinant human IL-10 protein standard (Cat. No. 554611).
Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines tested (e.g., mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).
Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISAreagents are not recommended for assaying serum samples. For testing human IL-10 in complex biological fluids like serum or plasma, our human IL-10 OptEIA™ ELISA Set (Cat. No. 555157) and OptEIA™ ELISA Kit (Cat. No. 550613) are recommended.
WB: The JES3-12G8 antibody has been reported to be useful for Western blotting.. Please note that this application is not routinely tested at BD Biosciences Pharmingen.