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Mouse Th1/Th2 Cytokine Cytometric Bead Array (CBA) Kit

BD™ Cytometric Bead Array (CBA) Mouse Th1/Th2 Cytokine Cytometric Bead Array (CBA) Kit

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BD™ Cytometric Bead Array (CBA)


The BD™ CBA Mouse Th1/Th2 Cyokine Kit (Catalog No. 551287) can be used to measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interferon-γ (IFN-γ), and Tumor Necrosis Factor (TNF) protein levels in a single sample. The kit performance has been optimized for analysis of physiologically relevant concentrations (pg/mL levels) of specific cytokine proteins in tissue culture supernatants and serum samples. The kit provides sufficient reagents for the quantitative analysis of 80 samples.


Vial LabelReagentQuantity
 A1 Mouse IL-2 Capture Beads 1 vial, 0.8 mL
 A2 Mouse IL-4 Capture Beads 1 vial, 0.8 mL
 A3 Mouse IL-5 Capture Beads 1 vial, 0.8 mL
 A4 Mouse IFN-γ Capture Beads 1 vial, 0.8 mL
 A5 Mouse TNF Capture Beads 1 vial, 0.8 mL
 B Mouse Th1/Th2 PE Detection Reagent 1 vial, 4 mL
 C Mouse Th1/Th2 Cytokine Standards 2 vials lyophilized
 D Cytometer Setup Beads 1 vial, 1.5 mL
 E PE Positive Control Detector 1 vial, 0.5 mL
 E2 FITC Positive Control Detector 1 vial, 0.5 mL
 F Wash Buffer 1 bottle, 130 mL
 G Assay Diluent 1 bottle, 30 mL


551287 Rev. 1
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Description Quantity/Size Part Number EntrezGene ID
Mouse IL-2 Capture Beads 0.8 mL (1 ea) 51-9005801 N/A
Mouse IL-4 Capture Beads 0.8 mL (1 ea) 51-9005803 N/A
Mouse IL-5 Capture Beads 0.8 mL (1 ea) 51-9005805 N/A
Mouse IFN-γ Capture Beads 0.8 mL (1 ea) 51-9005807 N/A
Mouse TNF Capture Beads 0.8 mL (1 ea) 51-9005809 N/A
B) Mouse Th1/Th2 PE Detection Reagent N/A 51-2523KC N/A
Mouse Th1/Th2 Cytokine Standards N/A 51-2524KC N/A
D Cytometer Setup Beads 1 Each (1 ea) 51-9005731 N/A
E1 PE Positive Control Detector 1 Each (1 ea) 51-9005732 N/A
E2 FITC Positive Control Detector 1 Each (1 ea) 51-9005734 N/A
F Wash Buffer 1 Each (1 ea) 51-2431KC N/A
G Assay Diluent 1 Each (1 ea) 51-2432KC N/A
551287 Rev. 1
Citations & References
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Development References (13)

  1. Bishop JE, Davis KA. A flow cytometric immunoassay for β2-microglobulin in whole blood. J Immunol Methods. 1997; 210:79–87. (Biology).
  2. Camilla C, Defoort JP, Delaage M, et al. A new flow cytometry-based multi-assay system 1, Application to cytokine immunoassays. Cytometry Suppl. 1998; 8:132. (Biology).
  3. Carson R, Vignali D. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999; 227:41–52. (Biology).
  4. Chen, R, L Lowe, et al. Simultaneous quantification of six human cytokines in a single sample using microparticle-based flow cytometric technology. Clin Chem. 1999; 9:1693-1694. (Biology).
  5. Collins DP, Luebering BJ, Shaut DM. T-lymphocyte functionality assessed by analysis of cytokine receptor expression, intracellular cytokine expression, and femtomolar detection of cytokine secretion by quantitative flow cytometry. Cytometry. 1998; 33:249-255. (Biology).
  6. Cook, EB, JL Stahl, et al. Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs non-allergics. J Immunol Methods. 2001; 254:109-118. (Biology).
  7. Dotti, G, B Salvodo, et al. Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells: A model for immunotherapy. Exp Hematol. 2001; 29:952-961. (Biology).
  8. Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettman JR Jr. Advanced multiplexed analysis with the FlowMetrix system. Clin Chem. 1997; 43:1749-1756. (Biology).
  9. Kricka LJ. Diamandis EP, Christopoulos TK, ed. Immunoassay. Academic Press; 1996:389-404.
  10. Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000; 39(4):250-259. (Biology). View Reference
  11. McHugh TM. Flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes. Methods Cell Biol. 1994; 33:613-629. (Biology). View Reference
  12. Oliver KG, Kettman JR, Fulton RJ. Multiplexed analysis of human cytokines by use of the FlowMetrix system. Clin Chem. 1998; 44:2057-2060. (Biology).
  13. Stall, A, Q Sun, et al. A single tube flow cytometric multibead assay for isotyping mouse monoclonal antibodies, Abstract LB77. Experimental Biology Meeting. 1998. (Biology).
View All (13) View Less
551287 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.