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Flow cytometric analysis of ROR2 expression in human chronic myelogenous leukemia cell line. K562 cells (ATCC CCL-243) were fixed in BD Cytofix™ fixation buffer (Cat. No. 554655) and permeabilized in BD Perm/Wash™ buffer (Cat. No. 554723). Permeablized cells were stained with either PE Mouse IgG1, k Isotype Control (Cat. No. 554680, dashed line) or PE Mouse Anti-ROR2 (Cat. No. 565551, solid line). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto™ II Cell Analyzer.
BD Pharmingen™ PE Mouse Anti-ROR2
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
The Ror2 monoclonal antibody specifically binds to the ST2 intracellular domain of ROR2 (Receptor tyrosine kinase-like Orphan Receptor 2). ROR2 is a type I transmembrane protein that belongs to a small family of receptor tyrosine kinases containing frizzled domains. Based on the homology of ROR2 and Wnt receptors Frz domains, ROR2 has been linked to Wnt signaling and embryonic development. Wnt5a-ROR2 signaling promotes osteoclastogenesis, and ROR2 is also critical for the formation of skeleton, heart, and genitals. In normal adult tissues, ROR2 is expressed in very low levels or undetectable. There is mounting evidence that ROR2 expression is associated with either tumor progression or suppression in different tissues. In humans, mutations in the ROR2 gene are associated with the autosomal recessive form of Robinow Syndrome and autosomal dominant Brachydactyly type B.
Development References (3)
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Ford CE, Qian Ma SS, Quadir A, Ward RL. The dual role of the novel Wnt receptor tyrosine kinase, ROR2, in human carcinogenesis. Int J Cancer. 2013; 133(4):779-787. (Biology). View Reference
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Mikels A, Minami Y, Nusse R. Ror2 receptor requires tyrosine kinase activity to mediate Wnt5A signaling. J Biol Chem. 2009; 284(44):30167-30176. (Immunogen: Immunohistochemistry, Western blot). View Reference
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van Amerongen R, Fuerer C, Mizutani M, Nusse R. Wnt5a can both activate and repress Wnt/β-catenin signaling during mouse embryonic development. Dev Biol. 2012; 369(1):101-114. (Clone-specific: Immunohistochemistry, Western blot). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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