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Flow cytometric analysis of CD318 expression on human colorectal adenocarcinoma cell line. HT-29 cells (ATCC HTB-38) were stained with PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; dashed line) or PE Mouse Anti-Human CD318 (CDCP1) (Cat. No. 566656; solid line) at 0.5 µg/test. Histogram plots were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Mouse Anti-Human CD318 (CDCP1)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The CUB1 monoclonal antibody specifically reacts with CUB domain-containing protein 1 (CDCP1 or CD318), which is expressed on primary or cultured pluripotent stem cells (eg ESC, iPSC, HSC, MSC, and NSC). It is not detected on normal mature hematopoietic cells of the peripheral blood, but it is found on leukemia and lymphoma cells. CDCP1 is a type I transmembrane protein with three extracellular CUB domains and five potential tyrosine phosphorylation sites in the cytoplasm. CUB (C1r/C1s, Uegf, Bmp) domains are ~110-amino-acid motifs almost exclusively found in developmentally regulated proteins that are involved in a variety of inter- or extracellular interactions. For example, CDCP1 is upregulated on some cancers and neoplastic cell lines and is involved in the control of tumor cell proliferation and metastasis.
Development References (5)
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Bühring HJ, Kuçi S, Conze T, et al. CDCP1 identifies a broad spectrum of normal and malignant stem/progenitor cell subsets of hematopoietic and nonhematopoietic origin. Stem Cells. 2004; 22(3):334-343. (Immunogen: Flow cytometry). View Reference
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Conze T, Lammers R, Kuci S, et al. CDCP1 is a novel marker for hematopoietic stem cells. Ann N Y Acad Sci. 2003; 996:222-226. (Clone-specific: Flow cytometry). View Reference
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Hooper JD, Zijlstra A, Aimes RT, et al. Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen. Oncogene. 2003; 22(12):1783-94. (Biology). View Reference
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Ikeda JI, Morii E, Kimura H, et al. Epigenetic regulation of the expression of the novel stem cell marker CDCP1 in cancer cells. J Pathol. 2006; 210(1):75-84. (Biology). View Reference
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Miyazawa Y, Uekita T, Hiraoka N, et al. CUB domain-containing protein 1, a prognostic factor for human pancreatic cancers, promotes cell migration and extracellular matrix degradation. Cancer Res. 2010; 70(12):5136-46. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.