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Product Details
BD OptiBuild™
MAIR-1; MAIR-I; LMIR1; CLM-8; Clm8; MMAC8; mcpir1; Pigr4
Mouse (Tested in Development)
Rat IgG2a, κ
Mouse CD300a Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
AB_2872535
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV786 under optimal conditions that minimize unconjugated dye and antibody.
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 786 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
- Cy is a trademark of GE Healthcare.
Companion Products
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.1 mg
Cat No.
553141
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Brilliant Stain Buffer RUO
Size
100 Tests
Cat No.
563794
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
BV786 Rat IgG2a, κ Isotype Control RUO
Size
50 µg
Cat No.
563335
748074 Rev. 2
Antibody Details
172224
The 172224 monoclonal antibody specifically recognizes CD300 antigen-like family member A (CD300a) which is also known as Myeloid-associated immunoglobulin-like receptor 1 (MAIR-1 or MAIR-I), Mast cell-derived paired immunoglobulin-like receptor 1 (Mcpir1), CMRF35-like molecule 8 (CLM-8 or Clm8), Leukocyte mono-Ig-like receptor 1 (Lmir1), or Inhibitory receptor protein 60 (IRp60). CD300a is a single-pass type I membrane protein that is encoded by the Cd300a gene which belongs to the immunoglobulin gene superfamily. The extracellular region of CD300a contains an IgV-type domain whereas its intracellular region has three ITIMs and a non-canonical ITIM. CD300a is expressed on some B cells, NK cells, macrophages, dendritic cells, neutrophils, eosinophils, and mast cells. CD300a functions as an inhibitory receptor that can downregulate the effector functions of the cells it is expressed on.
The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).
748074 Rev. 2
Format Details
BV786
The BD Horizon Brilliant Violet™ 786 (BV786) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an Ex Max of 407-nm and an acceptor dye with an Em Max at 786-nm. BV786, driven by BD innovation, is designed to be excited by the violet laser and detected using a filter, centered near 785 nm (e.g. 780/60 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and filters) are appropriate for this dye.
BV786
407 nm
786 nm
748074 Rev.2
Citations & References
View product citations for antibody "748074" on CiteAb
Development References (3)
-
Bachelet I, Munitz A, Mankutad D, Levi-Schaffer F. Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process.. J Biol Chem. 2006; 281(37):27190-6. (Biology). View Reference
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Carnec X, Meertens L, Dejarnac O, et al. The Phosphatidylserine and Phosphatidylethanolamine Receptor CD300a Binds Dengue Virus and Enhances Infection.. J Virol. 2016; 90(1):92-102. (Clone-specific: Flow cytometry). View Reference
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Kumagai H, Oki T, Tamitsu K, et al. Identification and characterization of a new pair of immunoglobulin-like receptors LMIR1 and 2 derived from murine bone marrow-derived mast cells.. Biochem Biophys Res Commun. 2003; 307(3):719-29. (Biology). View Reference
748074 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.