Skip to main content Skip to navigation
BV711 Mouse Anti-Mouse CD286 (TLR6)
Product Details
Down Arrow Up Arrow


BD OptiBuild™
Tlr6; Toll-like receptor 6
Mouse (Tested in Development)
Mouse BALB/c IgG1, κ
Mouse TLR6
Flow cytometry (Qualified)
0.2 mg/ml
21899
AB_2741391
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV711 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 711 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
  10. Cy is a trademark of GE Healthcare.
  11. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
743270 Rev. 1
Antibody Details
Down Arrow Up Arrow
C1N2

The C1N2 monoclonal antibody specifically binds to the Toll-like receptor 6 (TLR6), which is also known as CD286. CD286 belongs to the mammalian toll-like family of pattern recognition receptors (TLR) that participate in inducing innate immune responses to microbial pathogens. CD286 is a type I transmembrane glycoprotein comprised of an extracellular domain with a large number of leucinerich repeats (LRRs) and a cytoplasmic region with a signaling Toll/Interleukin-1 Receptor (TIR) domain. TLR6 is differentially expressed on the surface and intracellular compartments, including endolysosomes, of pre-B cells as well as immature or mature B cells. It is likewise expressed by monocytes, macrophages, or plasmacytoid, CD4+, or CD8+ dendritic cells. In association with TLR2 (CD282), CD286 recognizes microbial diacylated lipopeptides, lipoteichoic acid, and zymosan. The CD282/CD286 (TLR2/TLR60 heterodimer then transduces signaling responses leading to the production of proinflammatory cytokines by responding cells.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm.  BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter).  Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors.  However, the spillover can be corrected through compensation as with any other dye combination.

743270 Rev. 1
Format Details
Down Arrow Up Arrow
BV711
The BD Horizon Brilliant Violet™ 711 (BV711) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 713-nm. BV711, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 710-nm (e.g., a 712/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
BV711
Violet 405 nm
407 nm
713 nm
743270 Rev.1
Citations & References
Down Arrow Up Arrow
View product citations for antibody "743270" on CiteAb

Development References (5)

  1. Kang JY, Lee JO. Structural biology of the Toll-like receptor family.. Annu Rev Biochem. 2011; 80:917-41. (Biology). View Reference
  2. Marinho FA, de Paula RR, Mendes AC, et al. Toll-like receptor 6 senses Mycobacterium avium and is required for efficient control of mycobacterial infection.. Eur J Immunol. 2013; 43(9):2373-85. (Biology). View Reference
  3. Motoi Y, Shibata T, Takahashi K, et al. Lipopeptides are signaled by Toll-like receptor 1, 2 and 6 in endolysosomes.. Int Immunol. 2014; 26(10):563-73. (Immunogen: Flow cytometry, Immunoprecipitation). View Reference
  4. Takeuchi O, Kawai T, Sanjo H, et al. TLR6: A novel member of an expanding toll-like receptor family. Gene. 1999; 231(1-2):59-65. (Biology). View Reference
  5. de Almeida LA, Macedo GC, Marinho FA, et al. Toll-like receptor 6 plays an important role in host innate resistance to Brucella abortus infection in mice.. Infect Immun. 2013; 81(5):1654-62. (Biology). View Reference
View All (5) View Less
743270 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.