The C3 monoclonal antibody specifically reacts with epitope S of the rat MHC class I antigen, haplotypes RT1[a] (e.g., ACI, DA, COP) and RT1[b] (e.g., BP, BUF). It does not react with RT1[c] (e.g., AUG, PVG/c), RT1 (e.g., F344, LEW), or RT1[u] (e.g., AO, LOU, WF).
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.