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BD OptiBuild™ BV421 Rat Anti-Mouse IgG2a/IgG2b
Clone R2-40 (RUO)
Flow cytometric analysis using BD Pharmingen™ Purified Mouse Anti-Rat CD4 antibody (clone OX-35, left) or BD Pharmingen™ Mouse Anti-Rat CD45R antibody (clone HIS24, right) followed by BD OptiBuild™ BV421 Rat Anti-Mouse IgG2a/IgG2b antibody (Cat. No. 744292; solid line histogram) on live Lewis rat splenocytes, with secondary alone control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Flow cytometric analysis using BD Pharmingen™ Purified Mouse Anti-Rat CD4 antibody (clone OX-35, left) or BD Pharmingen™ Mouse Anti-Rat CD45R antibody (clone HIS24, right) followed by BD OptiBuild™ BV421 Rat Anti-Mouse IgG2a/IgG2b antibody (Cat. No. 744292; solid line histogram) on live Lewis rat splenocytes, with secondary alone control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Flow cytometric analysis using BD Pharmingen™ Purified Mouse Anti-Rat CD4 antibody (clone OX-35, left) or BD Pharmingen™ Mouse Anti-Rat CD45R antibody (clone HIS24, right) followed by BD OptiBuild™ BV421 Rat Anti-Mouse IgG2a/IgG2b antibody (Cat. No. 744292; solid line histogram) on live Lewis rat splenocytes, with secondary alone control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Flow cytometric analysis using BD Pharmingen™ Purified Mouse Anti-Rat CD4 antibody (clone OX-35, left) or BD Pharmingen™ Mouse Anti-Rat CD45R antibody (clone HIS24, right) followed by BD OptiBuild™ BV421 Rat Anti-Mouse IgG2a/IgG2b antibody (Cat. No. 744292; solid line histogram) on live Lewis rat splenocytes, with secondary alone control (dotted line histogram). Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Product Details
BD OptiBuild™
IgG2a (Ighg2a; Igh-1); IgG2b (Ighg2b; Igh-3; gamma2b)
Mouse (Tested in Development)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Pooled Mouse Ig
Flow cytometry (Qualified)
0.2 mg/ml
AB_2742122
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
Companion Products
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.5 mg
Cat No.
553142
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.1 mg
Cat No.
553141
Brilliant Stain Buffer RUO
Size
1000 Tests
Cat No.
566349
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Brilliant Stain Buffer RUO
Size
100 Tests
Cat No.
563794
744292 Rev. 2
Antibody Details
R2-40
The R2-40 monoclonal antibody specifically recognizes a common epitope, probably located in the CH1 domain, shared by mouse IgG2a, and IgG2b, of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
744292 Rev. 2
Format Details
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
744292 Rev.2
Citations & References
View product citations for antibody "744292" on CiteAb
Development References (2)
-
Benson MJ, Dillon SR, Castigli E, et al. Cutting edge: the dependence of plasma cells and independence of memory B cells on BAFF and APRIL.. J Immunol. 2008; 180(6):3655-9. (Clone-specific: Flow cytometry). View Reference
-
Chen CL, Lee CT, Liu YC, Wang JY, Lei HY, Yu CK. House dust mite Dermatophagoides farinae augments proinflammatory mediator productions and accessory function of alveolar macrophages: implications for allergic sensitization and inflammation.. J Immunol. 2003; 170(1):528-36. (Clone-specific: ELISA). View Reference
744292 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.