The MH7E7 monoclonal antibody specifically recognizes the extracellular domain of mouse Dendritic cell inhibitory receptor 4 (DCIR4). DCIR4 is a lectin receptor that is encoded by Clec4a1 (C-type lectin domain family 4, member a1) which belongs to the dendritic cell immunoreceptor (DCIR) family. DCIR4 (Clec4a1) is variably expressed on CD11b+ monocytes found in peripheral blood, secondary lymphoid tissues and the bone marrow. DCIR4 (Clec4a1) is reportedly expressed at low levels on Ly-6C-positive inflammatory monocytes and at high levels on Ly-6C-negative patrolling monocytes. It is not significantly expressed on eosinophils and neutrophils.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.