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Flow cytometric analysis of mouse CD282 expression by mouse spleen cells. C57BL/6 mouse splenic leucocytes were stained with PerCP-Cy™5.5 Rat Anti-Mouse CD11b antibody (Cat. No. 550993/561114) and either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; Left Plot) or BD Horizon BV421 Rat Anti-Mouse CD282 (TLR2) antibody (Cat. No. 565908; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD282 (TLR2) [or Ig Isotype control staining] versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.


BD Horizon™ BV421 Rat Anti-Mouse CD282 (TLR2)

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Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
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The CB225 monoclonal antibody specifically binds to the mouse Toll like receptor 2 (TLR2) which is also known as, CD282. CD282 is a type 1 transmembrane glycoprotein and TLR family member. TLR2 forms heterodimers with either TLR1 or TLR6, and is expressed on monocytes, macrophages, dendritic cells, granulocytes, endothelial cells, or epithelial cells. This pattern recognition receptor is important for sensing and transducing signals in response to microbial cell wall components including lipoproteins, peptidoglycans, and lipoteichoic acid. CD282 signals through intracellular MYD88 and TRAF6 leading to NF-kappa-B activation and the production of cytokines and inflammatory mediators by responding cells. TLR2 plays a major role in mediating inflammatory and innate immune responses to many microbial pathogens.

Development References (5)
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Heine H, Kirschning CJ, Lien E, Monks BG, Rothe M, Golenbock DT. Cutting edge: cells that carry A null allele for toll-like receptor 2 are capable of responding to endotoxin. J Immunol. 1999; 162(12):6971-6975. (Biology). View Reference
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Matsuguchi T, Takagi K, Musikacharoen T, Yoshikai Y. Gene expressions of lipopolysaccharide receptors, toll-like receptors 2 and 4, are differently regulated in mouse T lymphocytes. Blood. 2000; 95(4):1378-1385. (Biology). View Reference
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Motoi Y, Shibata T, Takahashi K, et al. Lipopeptides are signaled by Toll-like receptor 1, 2 and 6 in endolysosomes.. Int Immunol. 2014; 26(10):563-73. (Immunogen: Flow cytometry, Immunoprecipitation). View Reference
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Shibata T, Takemura N, Motoi Y, et al. PRAT4A-dependent expression of cell surface TLR5 on neutrophils, classical monocytes and dendritic cells. Int Immunol. 2012; 24(10):613. (Clone-specific: Flow cytometry). View Reference
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Underhill DM, Ozinsky A, Hajjar AM, et al. The Toll-like receptor 2 is recruited to macrophage phagosomes and discriminates between pathogens. Nature. 1999; 401(6755):811-815. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.