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BV421 Mouse Anti-Human HLA-DQ
BV421 Mouse Anti-Human HLA-DQ

Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human HLA-DQ antibody (Cat. No. 744763) on human peripheral blood. Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.

Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human HLA-DQ antibody (Cat. No. 744763) on human peripheral blood. Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.

Product Details
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BD OptiBuild™
MHC class II HLA-DQ; MHC HLA-DQ
Human (Tested in Development)
Mouse IgG1, κ
Tonsillar B lymphocytes (non-E-rosetting cells)
Flow cytometry (Qualified)
0.2 mg/ml
AB_2742463
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
744763 Rev. 2
Antibody Details
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SK10

The SK10 monoclonal antibody specifically recognizes a class II major histocompatibility complex (MHC) antigen, with a molecular weight of 26 to 34 kDa, distinct from HLA-DR3-5 and HLA-DP5 antigens. Anti-HLA-DQ recognizes a common polymorphic epitope present on HLA-DQ molecules of cells expressing DQw1 and DQw3 (usually associated with DR1, DR2, DR4, DR5, DRw8, DRw9, and DRw10) and absent from DQw2 (usually associated with DR3, and DR7). Therefore, the SK10 antibody not react with cells from DR3 or DR7 homozygotes or DR3/7 heterozygotes. The HLA-DQ antigen is present on approximately 10% of peripheral blood lymphocytes. HLA-DQ reacts weakly with most peripheral blood monocytes and mitogen-stimulated T-lymphocyte blasts. The SK10 antibody reacts with virtually all B-cell lines, some myelomas, and some myeloid leukemias, but only rarely with T-lymphocyte tumors.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

744763 Rev. 2
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
744763 Rev.2
Citations & References
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Development References (6)

  1. Brodsky FM. A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes.. Immunogenetics. 1984; 19(3):179-94. (Biology). View Reference
  2. Chen YX, Evans RL, Pollack MS, et al. Characterization and expression of the HLA-DC antigens defined by anti-Leu 10.. Hum Immunol. 1984; 10(4):221-35. (Clone-specific). View Reference
  3. Crumpton MF, Bodmer JG, Bodmer WF, Heyes JM, Lindsay J, Rudd CE. Biochemistry of class II antigens: workshop report. In: :29.
  4. Edwards JA, Durant BM, Jones DB, Evans PR, Smith JL. Differential expression of HLA class II antigens in fetal human spleen: relationship of HLA-DP, DQ, and DR to immunoglobulin expression.. J Immunol. 1986; 137(2):490-7. (Biology). View Reference
  5. Gonwa TA, Frost JP, Karr RW. All human monocytes have the capability of expressing HLA-DQ and HLA-DP molecules upon stimulation with interferon-gamma.. J Immunol. 1986; 137(2):519-24. (Biology). View Reference
  6. Robbins PA, Evans EL, Ding AH, Warner NL, Brodsky FM. Monoclonal antibodies that distinguish between class II antigens (HLA-DP, DQ, and DR) in 14 haplotypes.. Hum Immunol. 1987; 18(4):301-13. (Biology). View Reference
View All (6) View Less
744763 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.