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BD Horizon™ BUV395 Rat Anti-Mouse TER-119/Erythroid Cells
Clone TER-119 (RUO)
Two-color flow cytometric analysis of TER-119/Erythroid Cells expressed on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) antibody (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD45 antibody (Cat. No. 553081/561087) and either BD Horizon™ BUV395 Rat IgG2b, κ Isotype Control (Cat. No. 563560; Left Panel) or BD Horizon™ BUV395 Rat Anti-Mouse TER-119/Erythroid Cells antibody (Cat. No. 563827; Right Panel). Two-color flow cytometric dot plots showing the expressed levels of CD45 versus TER-119 (or Ig isotype control staining) were derived from gated events with the forward and side-scattered light characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.
Two-color flow cytometric analysis of TER-119/Erythroid Cells expressed on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) antibody (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD45 antibody (Cat. No. 553081/561087) and either BD Horizon™ BUV395 Rat IgG2b, κ Isotype Control (Cat. No. 563560; Left Panel) or BD Horizon™ BUV395 Rat Anti-Mouse TER-119/Erythroid Cells antibody (Cat. No. 563827; Right Panel). Two-color flow cytometric dot plots showing the expressed levels of CD45 versus TER-119 (or Ig isotype control staining) were derived from gated events with the forward and side-scattered light characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.
Two-color flow cytometric analysis of TER-119/Erythroid Cells expressed on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) antibody (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD45 antibody (Cat. No. 553081/561087) and either BD Horizon™ BUV395 Rat IgG2b, κ Isotype Control (Cat. No. 563560; Left Panel) or BD Horizon™ BUV395 Rat Anti-Mouse TER-119/Erythroid Cells antibody (Cat. No. 563827; Right Panel). Two-color flow cytometric dot plots showing the expressed levels of CD45 versus TER-119 (or Ig isotype control staining) were derived from gated events with the forward and side-scattered light characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.
Two-color flow cytometric analysis of TER-119/Erythroid Cells expressed on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) antibody (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD45 antibody (Cat. No. 553081/561087) and either BD Horizon™ BUV395 Rat IgG2b, κ Isotype Control (Cat. No. 563560; Left Panel) or BD Horizon™ BUV395 Rat Anti-Mouse TER-119/Erythroid Cells antibody (Cat. No. 563827; Right Panel). Two-color flow cytometric dot plots showing the expressed levels of CD45 versus TER-119 (or Ig isotype control staining) were derived from gated events with the forward and side-scattered light characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.
Product Details
BD Horizon™
Lymphocyte antigen 76; Ly76; Ly-76; TER-119; Ter119
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG2b, κ
Mouse Fetal Liver
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738438
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimum conditions, and unconjugated antibody and free BD Horizon BUV395 were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.1 mg
Cat No.
553141
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.5 mg
Cat No.
553142
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
PE Rat Anti-Mouse CD45 RUO
Size
0.1 mg
Cat No.
553081
BUV395 Rat IgG2b, κ Isotype Control RUO
Size
50 µg
Cat No.
563560
PE Rat Anti-Mouse CD45 RUO
Size
25 mg
Cat No.
561087
566206 Rev. 1
Antibody Details
TER-119
The TER-119 antibody specifically binds to a 52 kDa molecule associated with glycophorin A on cells of the erythroid lineage in embryonic yolk sac, fetal liver, newborn liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs. The TER-119 antigen is expressed on erythroid cells from pro-erythroblast through mature erythrocyte stages, but not on cells with BFU-E or CFU-E activities. The TER-119 epitope is not detected on hematopoietic stem cells, lymphoid cells, myeloid cells, or erythroleukemia lines. The TER-119 mAb is a component of the "lineage cocktail" used in studies of hematopoietic progenitors to detect, or deplete cells committed to the hematopoietic lineages.
The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355 nm laser equipped instrument. With an Ex Max at 348 nm and an Em Max at 395 nm, this dye has virtually no spillover into any other detector. BD Horizon™ BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.
566206 Rev. 1
Format Details
BUV395
The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BUV395
348 nm
395 nm
566206 Rev.1
Citations & References
View product citations for antibody "566206" on CiteAb
Development References (5)
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Ikuta K, Kina T, MacNeil I, et al. A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells. Cell. 1990; 62(5):863-874. (Clone-specific). View Reference
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Kina T, Ikuta K, Takayama E, et al. The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage. Br J Haematol. 2000; 109(2):280-287. (Immunogen: Immunoprecipitation, Western blot). View Reference
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Kitajima K, Kojima M, Nakajima K, et al. Definitive but not primitive hematopoiesis is impaired in jumonji mutant mice. Blood. 1999; 93(1):87-95. (Clone-specific: Flow cytometry, Immunohistochemistry). View Reference
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Maraskovsky E, Brasel K, Teepe M, et al. Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic cell subpopulations identified. J Exp Med. 1996; 184(5):1953-1962. (Clone-specific: Cell separation, Cytotoxicity, Depletion, Flow cytometry). View Reference
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Osawa M, Tokumoto Y, Nakauchi H. Hematopoietic stem cells. In: Herzenberg LA, Weir DM, Blackwell C, ed. Weir's Handbook of Experimental Immunology, 5th Edition. Cambridge: Blackwell Science; 1996:66.1-66.5.
566206 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.