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Biotin Rat Anti-Mouse IgG2a/2b
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Product Details
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BD Pharmingen™
IgG2a (Ighg2a; Igh-1); IgG2b (Ighg2b; Igh-3; gamma2b)
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Pooled Mouse Ig
ELISA (Routinely Tested), Flow cytometry (Tested During Development)
0.5 mg/ml
380793,380793, 16016
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

Biotin Rat Anti-Mouse IgG2a/2b may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgG2a/2b, we recommend FITC Rat Anti-Mouse IgG2a/2b (Cat. No. 553399). For immunohistochemical staining, we recommend the use of Biotin Rat Anti-Mouse IgG2a (Cat. No. 550332) or Biotin Rat Anti-Mouse IgG2b (Cat. No. 550333) in our special formulation for immunohistochemistry.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  4. Please refer to for technical protocols.
553398 Rev. 14
Antibody Details
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The R2-40 monoclonal antibody specifically recognizes a common epitope, probably located in the CH1 domain, shared by mouse IgG2a, and IgG2b, of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes.

553398 Rev. 14
Format Details
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
553398 Rev.14
Citations & References
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Development References (1)

  1. Benson MJ, Dillon SR, Castigli E, et al. Cutting edge: the dependence of plasma cells and independence of memory B cells on BAFF and APRIL.. J Immunol. 2008; 180(6):3655-9. (Biology). View Reference
553398 Rev. 14

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.