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Biotin Mouse Anti-Rabbit IgM
Product Details
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BD Pharmingen™
Rabbit (QC Testing)
Mouse IgG1, κ
Purified rabbit IgM
ELISA Detection (Routinely Tested), Flow cytometry (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

ELISA: For the rabbit IgM sandwich ELISA, purified clone 367.2 (available by custom order) is optimal for capture with Biotin Mouse Anti-Rabbit IgM for detection.

Flow cytometry: Biotin Mouse Anti-Rabbit IgM may be used as a primary reagent in immunofluorescent staining.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to to access safety data sheets (SDS).
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  5. Please refer to for technical protocols.
550938 Rev. 6
Antibody Details
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The 367.2 antibody reacts specifically with rabbit IgM.  It does not react with rabbit IgG or IgA isotypes.

550938 Rev. 6
Format Details
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
550938 Rev.6
Citations & References
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Development References (1)

  1. Vajdy M, Sethupathi P, Knight KL. Dependence of antibody somatic diversification on gut-associated lymphoid tissue in rabbits. J Immunol. 1998; 160(6):2725-2729. (Clone-specific: Flow cytometry). View Reference
550938 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.