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BD Pharmingen™ APC Mouse Anti-Human CD36
Clone CB38 (also known as NL07) (RUO)
Flow cytometric analysis of CD36 expression on human peripheral blood platelets. Platelets were stained with either APC Mouse IgM, κ Isotype Control (Cat. No. 555585; dashed line histogram) or APC Anti-Human CD36 antibody (Cat. No. 550956/561822; solid line histogram). The fluorescence histogram showing CD36 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact platelets. Flow cytometric analysis and data analysis was performed using a BD FACSCanto™ II System and FlowJo™ software.
Flow cytometric analysis of CD36 expression on human peripheral blood platelets. Platelets were stained with either APC Mouse IgM, κ Isotype Control (Cat. No. 555585; dashed line histogram) or APC Anti-Human CD36 antibody (Cat. No. 550956/561822; solid line histogram). The fluorescence histogram showing CD36 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact platelets. Flow cytometric analysis and data analysis was performed using a BD FACSCanto™ II System and FlowJo™ software.
Product Details
BD Pharmingen™
GPIIIb; Platelet GPIV; OKM5-antigen; PASIV
Human (QC Testing)
Mouse IgM, κ
Human Platelet Extracts
Flow cytometry (Routinely Tested)
20 µl
IV P106
AB_398480
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
561822 Rev. 9
Antibody Details
CB38
The CB38 monoclonal antibody specifically binds to CD36. CD36 is a 88 kDa glycoprotein IV (GPIV), the receptor for extracellular matrix proteins such as collagen and thrombospondin. CD36 is known to mediate the adhesion of Plasmodium falciparum. CD36 antigen is expressed on monocytes, platelets, endothelial cells, and some human tumor cell lines but not on lymphocytes and granulocytes. It is a very early marker of erythroid differentiation. CD36 antibody induces degranulation, release of ATP and serotonin, increase in [Ca2+]ι, and tyrosine phosphorylation of a substrate protein of 130 kDa.
561822 Rev. 9
Format Details
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
651 nm
660 nm
561822 Rev.9
Citations & References
View product citations for antibody "561822" on CiteAb
Development References (4)
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Alessio M, Ghigo D, Garbarino G, Geuna M, Malavasi F. Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07. Cell Immunol. 1991; 137(2):487-500. (Immunogen). View Reference
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Alessio M, Greco NJ, Primo L, et al. Platelet activation and inhibition of malarial cytoadherence by the anti-CD36 IgM monoclonal antibody NL07. Blood. 1993; 82(12):3637-3647. (Biology). View Reference
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Alessio M, Roggero S, Bussolino F, Saitta M, Malavasi F. Characterization of the murine monoclonal antibody NL07 specific for the human thrombospondin receptor (CD36 molecule). Curr Stud Hematol Blood Transfus. 1991; 58:182-186. (Biology). View Reference
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
561822 Rev. 9
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.