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BD Pharmingen™ APC-Cy™7 Rat Anti-Mouse Ig, κ Light Chain
Clone 187.1 (RUO)
Flow cytometric analysis of mouse Ig, κ light chain expressed on mouse splenocytes. Splenocytes from BALB/c mice were stained with a PE Rat anti-Mouse CD45R/B220 antibody (Cat. No. 553090/553089) and either with an APC-Cy™7 Rat IgG1, κ Isotype Control (Cat. No. 560534, Left Panel) or with the APC-Cy™7 Rat anti-Mouse Ig, κ Light Chain antibody (Cat. No. 561353, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of Ig, κ light chain (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Flow cytometric analysis of mouse Ig, κ light chain expressed on mouse splenocytes. Splenocytes from BALB/c mice were stained with a PE Rat anti-Mouse CD45R/B220 antibody (Cat. No. 553090/553089) and either with an APC-Cy™7 Rat IgG1, κ Isotype Control (Cat. No. 560534, Left Panel) or with the APC-Cy™7 Rat anti-Mouse Ig, κ Light Chain antibody (Cat. No. 561353, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of Ig, κ light chain (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Flow cytometric analysis of mouse Ig, κ light chain expressed on mouse splenocytes. Splenocytes from BALB/c mice were stained with a PE Rat anti-Mouse CD45R/B220 antibody (Cat. No. 553090/553089) and either with an APC-Cy™7 Rat IgG1, κ Isotype Control (Cat. No. 560534, Left Panel) or with the APC-Cy™7 Rat anti-Mouse Ig, κ Light Chain antibody (Cat. No. 561353, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of Ig, κ light chain (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Flow cytometric analysis of mouse Ig, κ light chain expressed on mouse splenocytes. Splenocytes from BALB/c mice were stained with a PE Rat anti-Mouse CD45R/B220 antibody (Cat. No. 553090/553089) and either with an APC-Cy™7 Rat IgG1, κ Isotype Control (Cat. No. 560534, Left Panel) or with the APC-Cy™7 Rat anti-Mouse Ig, κ Light Chain antibody (Cat. No. 561353, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of Ig, κ light chain (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Product Details
BD Pharmingen™
Ig kappa chain C region; IGKC; Igk-C; Ig, κ
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
Mouse IgG2b κ secreted by MPC-11 plasmacytoma
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10646040
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with APC-Cy7 under optimum conditions, and unconjugated antibody and free APC-Cy7 were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- APC-Cy7 is a tandem fluorochrome composed of Allophycocyanin (APC), which is excited by laser lines between 595 and 647 nm and serves as an energy donor, coupled to the cyanine dye Cy7™, which acts as an energy acceptor and fluoresces at 780 nm. BD Biosciences Pharmingen has maximized the fluorochrome energy transfer in APC-Cy7, thus maximizing its fluorescence emission intensity, minimizing residual emission from APC, and minimizing required electronic compensation in multilaser-laser flow cytometry systems. Note: Although every effort is made to minimize the lot-to-lot variation in residual emission from APC, it is strongly recommended that every lot be tested for differences in the amount of compensation required and that individual compensation controls are run for each APC-Cy7 conjugate.
- APC-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher.
- Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Warning: Some APC-Cy7 and PE-Cy7 conjugates show changes in their emission spectrum with prolonged exposure to formaldehyde. If you are unable to analyze fixed samples within four hours, we recommend that you use BD™ Stabilizing Fixative (Cat. No. 338036).
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
PE Rat Anti-Mouse CD45R/B220 RUO
Size
0.2 mg
Cat No.
553090
PE Rat Anti-Mouse CD45R/B220 RUO
Size
0.1 mg
Cat No.
553089
561353 Rev. 2
Antibody Details
187.1
The 187.1 monoclonal antibody specifically binds to kappa light chains of mouse immunoglobulins. The 187.1 antibody does not react with mouse λ1 or λ2 immunoglobulin lights chains or mouse immunoglobulin heavy chains.
561353 Rev. 2
Format Details
APC-Cy7
APC-Cy7 dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of a Allophycocyanin (APC) donor that has excitation maxima (Ex Max) of 651 nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 779 nm. APC-Cy7 can be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 780 nm (e.g., a 760/60 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC-Cy7
651 nm
779 nm
561353 Rev.2
Citations & References
View product citations for antibody "561353" on CiteAb
Development References (1)
-
Yelton DE, Desaymard C, Scharff MD. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma. 1981; 1(1):5-11. (Immunogen). View Reference
561353 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.