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Multiparameter flow cytometric analysis of TLR5 (CD285) expression on human peripheral blood leucocytes. Whole blood was stained with FITC Mouse Anti-Human CD14 antibody (Cat. No. 555397/557153/561712) and either Alexa Fluor® 647 mouse IgG1, κ Isotype Control [Cat. No. 557714; Left Panels] or Alexa Fluor® 647 Mouse Anti-Human TLR5 (CD285) antibody [Cat. No. 564344; Right Panels]. The erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric contour plots showing the correlated expression of CD14 [Upper Panels] versus TLR5 (CD285) [or Ig Isotype control staining] were derived from gated events with the light-scatter characteristics of viable mononuclear leucocytes. Two-parameter flow cytometric contour plots showing the correlated Side Light Scatter (SSC) characteristics [Lower Panels] versus TLR5 (CD285) expression were derived from gated events with the light-scatter characteristics of total viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.


BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human TLR5 (CD285)

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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The 624915 monoclonal antibody specifically binds to TLR5 (Toll-like Receptor 5) which is also known as CD285, MELIOS, SLEB1, and TIL3. TLR5 is a type I transmembrane glycoprotein of the Toll-like Receptor family. It is expressed on mucosal epithelial cells and leucocytes, including monocytes and some immature dendritic cells and CD4-positive T cells. CD285 binds to bacterial flagellin. Flagellin binding induces TLR5 dimerization and intracellular signaling through MyD88 resulting in the cellular production of proinflammatory cytokines and immune system activation.
Development References (3)
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Aderem A, Ulevitch RJ. Toll-like receptors in the induction of the innate immune response. Nature. 2000; 406(6797):782-787. (Biology). View Reference
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Bates JT, Graff AH, Phipps JP, Grayson JM, Mizel SB. Enhanced antigen processing of flagellin fusion proteins promotes the antigen-specific CD8+ T cell response independently of TLR5 and MyD88. J Immunol. 2011; 186(11):6255-6262. (Biology). View Reference
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Smith KD, Andersen-Nissen E, Hayashi F, et al. Toll-like receptor 5 recognizes a conserved site on flagellin required for protofilament formation and bacterial motility. Nat Immunol. 2003; 4(12):1247-1253. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.