BD Pharmingen™ PE Mouse Anti-Human CD68 Set
Description
Reacts with a 110 kDa-type I-transmembrane glycoprotein expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, granulocytes, myeloid progenitor cells and, reportedly, a subset of CD34+ hemopoietic bone marrow progenitor cells. CD68 belongs to the sialomucin family and its function has not been fully elucidated. This antibody could be useful in studies of myeloid cell development.
Preparation And Storage
Recommended Assay Procedures
We recommend the use of Cytofix/Cytoperm (Cat. No. 554714) for the cells fixation and permeabilization, and then do the intracellular staining.
Product Notices
- This antibody has been optimized and preassayed with its matched isotype control to be used at the recommended volume of 20 ul/test. Titration of the reagents or substituting with other (non-matched) isotype control is NOT recommended.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| PE Mouse Anti-Human CD68 | 100 Tests (1 ea) | 51-36805X | N/A |
| PE Mouse IgG2b κ Isotype Control | 100 Tests (1 ea) | 51-35425X | N/A |
Development References (4)
-
Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
-
Davey FR, Cordell JL, Erber WN, Pulford KA, Gatter KC, Mason DY. Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages. J Clin Pathol. 1988; 41(7):753-758. (Biology). View Reference
-
Davey FR, Erber WN, Gatter KC, Mason DY. The use of monoclonal antibody Y1/82A in the identification of acute myeloblastic and monocytic leukemias. Am J Clin Pathol. 1988; 89(1):76-80. (Biology). View Reference
-
Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.