BD Pharmingen™ NHP T/B/NK Cell Cocktail
Three-color analysis of the expression of CD3, CD16, and CD20 on lysed whole blood from Rhesus monkey. PBMC from Rhesus monkey were stained with either Isotype Control Cocktail C (Cat. no. 558659; data not shown) or NHP T/B/NK Lymphocyte Cocktail (Cat. no. 558639). During data analysis, lymphocytes were identified by scatter profile and CD45 expression. The figure on the left represents the CD3 and CD16 profile while the figure on the right represents the CD3 and CD20 profile. Flow cytometry was performed on a BD FACSCalibur™.
Three-color analysis of the expression of CD3, CD16, and CD20 on lysed whole blood from Rhesus monkey. PBMC from Rhesus monkey were stained with either Isotype Control Cocktail C (Cat. no. 558659; data not shown) or NHP T/B/NK Lymphocyte Cocktail (Cat. no. 558639). During data analysis, lymphocytes were identified by scatter profile and CD45 expression. The figure on the left represents the CD3 and CD16 profile while the figure on the right represents the CD3 and CD20 profile. Flow cytometry was performed on a BD FACSCalibur™.
Description
The NHP T/B/NK Cocktail is a three-color reagent cocktail designed to identify NHP T, B, and NK lymphocyte populations by direct immunofluorescent staining with flow cytometric analysis. Clone SP34-2 is a mouse IgG1 isotype monoclonal antibody, descendant of SP34 (mouse IgG3), with the same specificity and reactivity pattern as the parent clone. It cross-reacts with a major subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, and rhesus, cynomolgus, and pigtail macaque monkeys. The monoclonal antibody clone L27 reacts with CD20 found only on B lymphocytes. The monoclonal antibody clone 3G8 reacts with CD16, the 50-65 kDa transmembrane form of IgG Fc (FcγRIII), a human NK-cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Companion Products
| Description | Quantity/Size | Part Number | Clone | Isotype |
|---|---|---|---|---|
| FITC Mouse anti-Human CD3 | N/A | 51-540-9004931 | SP34-2 | IgG1, λ |
| PE Mouse Anti-Human CD16 | N/A | 51-550-30625 | 3G8 | IgG1, κ |
| APC Mouse anti-Human CD20 | N/A | 51-590-9007504 | L27 | IgG1, κ |
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.