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Dog Compensation Set

BD Pharmingen™ Dog Compensation Set

Product Details
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BD Pharmingen™
Flow cytometry (Routinely Tested)


The Dog Compensation Set is intended to be used with the Dog T lymphocyte Cocktail (Cat. No. 558699) or the Dog Activated T Lymphocyte Cocktail (Cat. No. 558704) for instrument compensation.  Compensation is the process by which the effect of spectral overlap among flurochromes can be corrected, minimizing fluorescence signal spillover in neighboring channels.  Traditionally, compensation values are entered into software programs allowing for the adjustment among the overlapping flurochrome emissions.  These values can be achieved using single color controls, and verified using fluorescence-minus-one controls, or ad-mixtures of single color controls.  Use this set to properly adjust your FITC/PE/APC compensation matrix for the Dog T Lymphocyte or Dog Activated T Lymphocyte Cocktails.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  6. Please refer to for technical protocols.
  7. Please refer to to access safety data sheets (SDS).
558715 Rev. 4
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Description Quantity/Size Part Number EntrezGene ID
PE Mouse anti-Dog CD4 N/A 51-9005079 N/A
APC Mouse anti-Dog Pan T Cell N/A 51-9005081 N/A
FITC Mouse anti-Dog CD8 N/A 51-9005080 N/A
FITC Mouse anti-Dog Activated T Cells N/A 51-9005083 N/A
558715 Rev. 4
Citations & References
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Development References (3)

  1. Cobbold S, Metcalfe S. Monoclonal antibodies that define canine homologues of human CD antigens: Summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 1994; 43:137-154. (Clone-specific). View Reference
  2. Gebhard GH, Carter PB. Identification of canine T-lymphocyte subsets with monoclonal antibodies. Vet Immunol Immunopathol. 1992; 33:187-199. (Biology). View Reference
  3. Shapiro HM. Practical flow cytometry, 3rd ed.. New York: Wiley-Liss; 1995:18-19.
558715 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.