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APC Mouse Anti-Human CD8β
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BD FastImmune™
CD8B; CD8B1; CD8 beta; CD8β; Leu2; Ly3; LYT3
Human
Mouse BALB/c IgG2a, κ
CD8+ suppressor T-cell
Flow cytometry
25 μg/mL
5 μL
AB_1645723
RUO (GMP)


641058 Rev. 1
Antibody Details
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2ST8.5H7

The CD8β antibody, clone 2ST8.5H7, is derived from the hybridization NS-1 mouse tumor cells with splenocytes from Balb/c mice immunized with CD8+ suppressor T-cell clone termed 20a6.

The CD8β antibody recognizes an epitope formed by the combination of CD8 alpha and beta chains. The majority of peripheral blood CD8+ T lymphocytes expresses an α/β heterodimer (32, 30 kilodaltons (kDa)), while CD8+CD16+ natural killer (NK) lymphocytes and CD8+ T-cell receptor (TCR)-γ/δ+ T lymphocytes express only an α/α homodimer. CD8β can therefore be used to selectively bind to CD8+ T cells while excluding CD8+ NK cells. The CD8 antigenic determinant binds to class I major histocompatibility (MHC) molecules, resulting in increased adhesion between the CD8+ T lymphocytes and target cells. Binding of the CD8 antigen to class I MHC molecules enhances the activation of resting T lymphocytes. The CD8 antigen is coupled to a protein tyrosine kinase, p56lck. The CD8:p56lck complex can play a role in T-lymphocyte activation through mediation of the interactions between the CD8 antigen and the CD3/TCR complex.

641058 Rev. 1
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
641058 Rev.1
Citations & References
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View product citations for antibody "641058" on CiteAb

Development References (30)

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View All (30) View Less
641058 Rev. 1

 

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