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      APC Mouse Anti-Human CD154 (CD40L)
      Product Details
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      BD™
      CD40LG; CD40 ligand; CD40L; gp39; TNFSF5; T-BAM; TRAP
      Human
      Mouse BALB/c IgG1, κ
      sgp39-CD8 and Activated Human PBL
      Flow cytometry
      100 μg/mL
      5 μL
      959
      RUO (GMP)


      648887 Rev. 1
      Antibody Details
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      89-76

      CD154, clone 89-76, is derived from hybridization of mouse NS-1 cells with spleen cells from BALB/c mice immunized with sgp39-CD8 and activated human peripheral blood lymphocytes (PBLs).

      CD154 (CD40 ligand or CD40L) recognizes a 39-kilodalton (kDa) antigen that is a member of the tumor necrosis factor (TNF) receptor family. CD154 is transiently expressed on activated CD4 T lymphocytes. The expression of CD154 by activated T-helper cells triggers B-cell cycling through binding to CD40. This co-receptor interaction is required for B-lymphocyte maturation, response to T-dependent ligands, and isotype switching. T-lymphocyte proliferation and generation of cytotoxic T lymphocytes (CTLs) is associated with the presence of CD154. Defects in the CD154 gene are associated with the X-linked hyper IgM syndrome.

      648887 Rev. 1
      Format Details
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      APC
      Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      APC
      Red 627-640 nm
      651 nm
      660 nm
      648887 Rev.1
      Citations & References
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      View product citations for antibody "648887" on CiteAb

      Development References (23)

      1. Armitage RJ, Fanslow WC, Strockbine L, et al. Molecular and biological characterization of a murine ligand for CD40.. Nature. 1992; 357(6373):80-2. (Biology). View Reference
      2. Aruffo A, Farrington M, Hollenbaugh D, et al. The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.. Cell. 1993; 72(2):291-300. (Biology). View Reference
      3. Ashokkumar C, Talukdar A, Sun Q, et al. Allospecific CD154+ T cells associate with rejection risk after pediatric liver transplantation.. Am J Transplant. 2009; 9(1):179-91. (Biology). View Reference
      4. Axcrona K, Gray D, Leanderson T. Regulation of B cell growth and differentiation via CD21 and CD40. Eur J Immunol. 1996; 26(9):2203-2207. (Biology). View Reference
      5. Bour-Jordan H, Bluestone JA. Regulating the regulators: costimulatory signals control the homeostasis and function of regulatory T cells.. Immunol Rev. 2009; 229(1):41-66. (Biology). View Reference
      6. Carbone A, Gloghini A, Gruss HJ, Pinto A. CD40 ligand is constitutively expressed in a subset of T cell lymphomas and on the microenvironmental reactive T cells of follicular lymphomas and Hodgkin's disease.. Am J Pathol. 1995; 147(4):912-22. (Biology). View Reference
      7. Cayabyab M, Phillips JH, Lanier LL. CD40 preferentially costimulates activation of CD4+ T lymphocytes.. J Immunol. 1994; 152(4):1523-31. (Biology). View Reference
      8. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
      9. Chattopadhyay PK, Yu J, Roederer M. A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles.. Nat Med. 2005; 11(10):1113-7. (Biology). View Reference
      10. Clinical and Laboratory Standards Institute. 2005. (Biology).
      11. Ding L, Green JM, Thompson CB, Shevach EM. B7/CD28-dependent and -independent induction of CD40 ligand expression.. J Immunol. 1995; 155(11):5124-32. (Biology). View Reference
      12. Durie FH, Foy TM, Masters SR, Laman JD, Noelle RJ. The role of CD40 in the regulation of humoral and cell-mediated immunity.. Immunol Today. 1994; 15(9):406-11. (Biology). View Reference
      13. Frentsch M, Arbach O, Kirchhoff D, et al. Direct access to CD4+ T cells specific for defined antigens according to CD154 expression.. Nat Med. 2005; 11(10):1118-24. (Biology). View Reference
      14. Fuleihan R, Ahern D, Geha RS. CD40 ligand expression is developmentally regulated in human thymocytes.. Clin Immunol Immunopathol. 1995; 76(1 Pt 1):52-8. (Biology). View Reference
      15. Gauchat JF, Aubry JP, Disanto J, et al. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1995:416-418.
      16. Han S, Hathcock K, Zheng B, Kepler TB, Hodes R, Kelsoe G. Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers.. J Immunol. 1995; 155(2):556-67. (Biology). View Reference
      17. Hollenbaugh D, Grosmaire LS, Kullas CD, et al. The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity.. EMBO J. 1992; 11(12):4313-21. (Biology). View Reference
      18. Lane P, Traunecker A, Hubele S, Inui S, Lanzavecchia A, Gray D. Activated human T cells express a ligand for the human B cell-associated antigen CD40 which participates in T cell-dependent activation of B lymphocytes.. Eur J Immunol. 1992; 22(10):2573-8. (Biology). View Reference
      19. Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc Natl Acad Sci U S A. 1992; 89(14):6550-6554. (Biology). View Reference
      20. Noelle RJ. The role of gp39 (CD40L) in immunity.. Clin Immunol Immunopathol. 1995; 76(3 Pt 2):S203-7. (Biology). View Reference
      21. Ramesh N, Morio T, Fuleihan R, et al. CD40-CD40 ligand (CD40L) interactions and X-linked hyperIgM syndrome (HIGMX-1).. Clin Immunol Immunopathol. 1995; 76(3 Pt 2):S208-13. (Biology). View Reference
      22. Roy M, Waldschmidt T, Aruffo A, Ledbetter JA, Noelle RJ. The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells. J Immunol. 1993; 151(5):2497-2510. (Biology). View Reference
      23. Spriggs MK, Armitage RJ, Strockbine L, et al. Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion.. J Exp Med. 1992; 176(6):1543-50. (Biology). View Reference
      View All (23) View Less
      648887 Rev. 1

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      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

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      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.