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Purified Mouse Anti-Mouse CD247
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BD Pharmingen™
CD3ζ chain
Mouse (QC Testing)
Mouse IgG2b
Mouse CD3ζ C-terminus synthetic peptide
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
32 kDa
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.


Applications include western blot analysis (1-2 µg/ml) and immunoprecipitation (1-2 µg/1x10 [6] cells).  Immunoprecipitation application is not routinely tested, only tested during development.


  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554241 Rev. 8
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The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multicomponent complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes, Ti is present as a disulfide-linked α-β heterodimer. A minor fraction express Ti as a γ-δ heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains: γ, δ, ε, ζ, and η. The relative masses of the mouse CD3 chains are 21, 28, 26, 32, (dimer) and 21 kDa, respectively. Clone 1ζ3A1 recognizes an epitope in the C-terminus of mouse CD3ζ. A synthetic peptide corresponding to the C-terminus of mouse CD3ζ (BSACDTYDALHMQTLAPR) was used as immunogen. Hybridomas were derived from Balb/c spleen cells x NS-1.

554241 Rev. 8
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
554241 Rev.8
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研发参考 (1)

  1. Hussey RE, Clayton LK, Diener A, et al. Overexpression of CD3 eta during thymic development does not alter the negative selection process. J Immunol. 1993; 150(4):1183-1194. (Immunogen: Immunoprecipitation, Western blot). 查看参考
554241 Rev. 8

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.