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Purified Mouse Anti-Human DP103
Purified Mouse Anti-Human DP103
Western blot analysis of DP103 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-human DP103 antibody.
Western blot analysis of DP103 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-human DP103 antibody.
商品详情
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BD Transduction Laboratories™
Gemin3; DEAD box Protein-103; Ddx20
Human (QC Testing)
Mouse IgG2b
Human DP103 aa. 667-783
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
103 kDa
250 µg/ml
AB_399524
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612152 Rev. 1
抗体详情
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2/DP103/Gemin3

DEAD box proteins, a family of putative RNA helicases, are characterized by eight conserved amino acid motifs that are arranged in a core region as found in the prototypical member of the family, eIF-4A. The family's name is derived from the amino acid sequence Asp-Glu-Ala-Asp (DEAD) that is located within the ATP hydrolysis motif. DEAD box proteins have been implicated in translation initiation and RNA splicing, degradation, and stability. The DEAD box protein, DP103, contains seven N-terminal helicase motifs characteristic of DEAD box proteins followed by an SMN interaction domain (SID). DP103 mRNA has been reported to be widely expressed, and DP103 protein is found in the nucleus and cytoplasm. SMN, the gene mutated in spinal muscular atrophy, forms a 20S nuclear complex that includes DP103, SIP1 (SMN-interacting protein 1), and snRNPs. DP103 also co-localizes with SMN and SIP1 to nuclear bodies called gems. In addition, DP103 interacts with the proximal repressor domain of steroidogenic factor-1, a nuclear receptor essential for development of the gonads, adrenal gland, and hypothalamic nuclei. Thus, DP103 may have roles in SMN complex modulation of RNA splicing and in transcriptional repression.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612152 Rev. 1
格式详情
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612152 Rev.1
报价单和参考
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研发参考 (4)

  1. Charroux B, Pellizzoni L, Perkinson RA, Shevchenko A, Mann M, Dreyfuss G. Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems. J Cell Biol. 1999; 147(6):1181-1194. (Biology). 查看参考
  2. Grundhoff AT, Kremmer E, Tureci O, et al. Characterization of DP103, a novel DEAD box protein that binds to the Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C. J Biol Chem. 1999; 274(27):19136-19144. (Biology). 查看参考
  3. Meister G, Buhler D, Laggerbauer B, Zobawa M, Lottspeich F, Fischer U. Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins. Hum Mol Genet. 2000; 9(13):1977-1986. (Biology). 查看参考
  4. Ou Q, Mouillet JF, Yan X, Dorn C, Crawford PA, Sadovsky Y. The DEAD box protein DP103 is a regulator of steroidogenic factor-1. Mol Endocrinol. 2001; 15(1):69-79. (Biology: Western blot). 查看参考
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612152 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.