Purified Mouse Anti-SIII p15

BD Transduction Laboratories™ Purified Mouse Anti-SIII p15

Name: Purified Mouse Anti-SIII p15
Brand: BD Transduction Laboratories™
SKU: 610761

克隆 56/SIIIp15

(RUO)

Western blot analysis of Elongin C/SIII p15 on HepG2 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Elongin C/SIII p15 antibody.

Immunofluorescent staining of Human Fibroblasts.

Western blot analysis of Elongin C/SIII p15 on HepG2 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Elongin C/SIII p15 antibody.

Immunofluorescent staining of Human Fibroblasts.

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协议库

科学资源

商品详情

Brand: BD Transduction Laboratories™

Reactivity: Human (QC Testing), Mouse, Rat, Dog, Frog (Tested in Development)

Isotype: Mouse IgG1

Immunogen: Human SIII p15 aa. 1-112

Application: Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)

Target Molecular Weight: 15 kDa

Concentration: 250 µg/ml

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

商品通知

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

610761 Rev. 1

抗体详情

56/SIIIp15

SIII p15 is one of three polypeptides that comprise the approximate 140kDa RNA polymerase II transcription factor SIII. The other two polypeptides have the molecular weights of 110kDa and 18kDa. All three polypeptides are required for transcription factor SIII function. Although the polypeptides were identified and purified from rat liver nuclear extracts, tissue expression of this transcription factor appears to be widespread. RNA polymerase II recognizes the SIII transcription factor and in turn initiates transcription from these correctly initiated transcripts. A yeast transcription factor designated YES may be the yeast homolog to SIII.

610761 Rev. 1

格式详情

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

格式: Purified

610761 Rev.1

报价单和参考

研发参考 (2)

Bradsher JN, Jackson KW, Conaway RC, Conaway JW. RNA polymerase II transcription factor SIII. I. Identification, purification, and properties. J Biol Chem. 1993; 268(34):25587-25593. (Biology). 查看参考
Kuznetsova AV, Meller J, Schnell PO. von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif and targets it for ubiquitination. Proc Natl Acad Sci U S A. 2003; 100(5):2706-2711. (Clone-specific: Western blot). 查看参考

610761 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

BD Transduction Laboratories™ Purified Mouse Anti-SIII p15

准备和存储

推荐的实验流程

商品通知

研发参考 (2)