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      Purified Mouse Anti-Phosphotyrosine
      Purified Mouse Anti-Phosphotyrosine
      Western blot analysis of Phosphotyrosine on A431 lysate. 1:1000 (lane 1), 1:2000 (lane 2), 1:4000 (lane 3) dilution of Phosphotyrosine.
      Purified Mouse Anti-Phosphotyrosine

      Phosphotyrosine, clone PY69. Left panel: A431 control. Right panel: A431 + EGF

      Purified Mouse Anti-Phosphotyrosine Purified Mouse Anti-Phosphotyrosine
      Western blot analysis of Phosphotyrosine on A431 lysate. 1:1000 (lane 1), 1:2000 (lane 2), 1:4000 (lane 3) dilution of Phosphotyrosine.

      Phosphotyrosine, clone PY69. Left panel: A431 control. Right panel: A431 + EGF

      商品详情
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      BD Transduction Laboratories™
      Human (QC Testing), Mouse, Rat, Chicken, Dog, Frog (Tested in Development)
      Mouse IgG2a
      Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
      1.0 mg/ml
      AB_397806
      Aqueous buffered solution containing glycerol and ≤0.09% sodium azide.
      RUO


      推荐的实验流程

      The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes.

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      商品通知

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      610430 Rev. 1
      抗体详情
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      PY69

      Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.

      610430 Rev. 1
      格式详情
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610430 Rev.1
      报价单和参考
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      研发参考 (5)

      1. Doronin S, Shumay E, Wang HY, Malbon CC. Akt mediates sequestration of the beta(2)-adrenergic receptor in response to insulin. J Biol Chem. 2002; 277(17):15124-15131. (Clone-specific: Western blot). 查看参考
      2. Doronin S, Wang Hy HY, Malbon CC. Insulin stimulates phosphorylation of the beta 2-adrenergic receptor by the insulin receptor, creating a potent feedback inhibitor of its tyrosine kinase. J Biol Chem. 2002; 277(12):10698-10703. (Clone-specific: Western blot). 查看参考
      3. Glenney JR Jr, Zokas L, Kamps MP. Monoclonal antibodies to phosphotyrosine. J Immunol Methods. 1988; 109(2):277-285. (Biology). 查看参考
      4. Moro L, Dolce L, Cabodi S, et al. Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p13Cas and leads to phosphorylation of specific EGF receptor tyrosines. J Biol Chem. 2002; 277(11):9405-9414. (Clone-specific: Western blot).
      5. Yan Z, Deng X, Friedman E. Oncogenic Ki-ras confers a more aggressive colon cancer phenotype through modification of transforming growth factor-beta receptor III. J Biol Chem. 2001; 276(2):1555-1563. (Clone-specific: Western blot). 查看参考
      查看所有文件 (5) 查看更少内容
      610430 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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