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Purified Mouse Anti-Human IκBα
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Purified Mouse Anti-Human IκBα
Western blot of IκBα on human endothelial lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of IκBα.
Purified Mouse Anti-Human IκBα

Immunofluroescent staining of MCF7 cells with anti-IκBα antibody.

Western blot of IκBα on human endothelial lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of IκBα.

Immunofluroescent staining of MCF7 cells with anti-IκBα antibody.

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BD Transduction Laboratories™
MAD-3; I-kappa-B-alpha; IkB-alpha; NFKBIA; NF-kappa-B inhibitor alpha
Human (QC Testing)
Mouse IgG1
Human IκBα aa. 145-302 Recombinant Protein
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
38 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.


  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610690 Rev. 1
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NF-κB is a transcription factor that is a member of the mammalian NF-κB/Rel family of proteins. Members of this family are involved in the regulation of cell proliferation, immune function, as well as development. In resting cells, IκBα binds to and maintains NF-κB in the cytoplasm by blocking the nuclear localization sequences of NF-κB. In the cellular response to an extracellular signal, IκBα is phosphorylated and subsequently degraded via the ubiquination-proteasome pathway, allowing NF-κB to translocate to the nucleus. Once in the nucleus, NF-κB can induce the transcription of IκBα thereby renewing the cycle so that IκBα can form a complex with NF-κB and maintain it in its cytoplasmic location. IκBα -/- mice show an increased level of NF-κB activity and have been shown to die soon after birth.

The 25/IkBa/MAD-3 monoclonal antibody recognizes human IκBα regardless of phosphorylation status and does not cross-react with mouse IκBα.

610690 Rev. 1
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610690 Rev.1
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研发参考 (5)

  1. Auphan N, DiDonato JA, Rosette C, Helmberg A, Karin M. Immunosuppression by glucocorticoids: inhibition of NF-kappa B activity through induction of I kappa B synthesis. Science. 1995; 270(5234):286-290. (Biology). 查看参考
  2. Cordle SR, Donald R, Read MA, Hawiger J. Lipopolysaccharide induces phosphorylation of MAD3 and activation of c-Rel and related NF-kappa B proteins in human monocytic THP-1 cells. J Biol Chem. 1993; 268(16):11803-11810. (Biology). 查看参考
  3. Haskill S, Beg AA, Tompkins SM, et al. Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity. Cell. 1991; 65(7):1281-1289. (Biology). 查看参考
  4. Nakashio A, Fujita N, Rokudai S, Sato S, Tsuruo T. Prevention of phosphatidylinositol 3'-kinase-Akt survival signaling pathway during topotecan-induced apoptosis. Cancer Res. 2000; 60(18):5303-5309. (Clone-specific: Western blot). 查看参考
  5. Traenckner EB, Pahl HL, Henkel T, Schmidt KN, Wilk S, Baeuerle PA. Phosphorylation of human I kappa B-alpha on serines 32 and 36 controls I kappa B-alpha proteolysis and NF-kappa B activation in response to diverse stimuli. EMBO J. 1995; 14(12):2876-2883. (Biology). 查看参考
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610690 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.