准备和存储
推荐的实验流程
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
商品通知
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
配套商品
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With the exception of Hsp110, the stress or heat shock proteins have been thoroughly characterized. Hsp110 has been reported to be expressed in most tissues. Like other stress proteins, Hsp110 is induced by heat shock and its induction is associated with thermotolerance. The sequence of Hsp110 reportedly bears similarity to several Hsp70-related proteins that have been termed Hsp110/SSE. The carboxy-terminal domains of these proteins and Hsp110 contain a putative peptide-binding site. These proteins also contain six highly conserved regions found in the same progression, as well as five conserved ATP-binding motifs.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
研发参考 (2)
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Lee-Yoon D, Easton D, Murawski M, Burd R, Subjeck JR. Identification of a major subfamily of large hsp70-like proteins through the cloning of the mammalian 110-kDa heat shock protein. J Biol Chem. 1995; 270(26):15725-15733. (Biology). 查看参考
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Moore JK, Scheinman RI, Bellgrau D. The identification of a novel T cell activation state controlled by a diabetogenic gene. J Immunol. 2001; 166(1):241-248. (Biology: Western blot). 查看参考
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