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Purified Mouse Anti-AKAP79
Purified Mouse Anti-AKAP79
Western blot analysis of AKAP79 on SW13 lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-AKAP79.
Purified Mouse Anti-AKAP79

Immunofluorescent staining of endothelial cells.

Western blot analysis of AKAP79 on SW13 lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-AKAP79.

Immunofluorescent staining of endothelial cells.

商品详情
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human AKAP79 aa. 180-427
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
79 kDa
250 µg/ml
AB_397706
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


商品通知

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610314 Rev. 1
抗体详情
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22/AKAP79

The type II cAMP-dependent Protein Kinase (PKA) is compartmentalized within the cell. To maintain this localization of type II PKAs, the regulatory subunit  (RII) interacts with specific RII-anchoring proteins. For instance, attachment of type II PKA to the cytoskeleton occurs through the binding of RII to microtubule-associated protein 2 (MAP2). In brain, several proteins have been identified as PKA type II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP79 is a 79kDa human RII-anchoring protein. AKAP, PKA type II, and calcineurin (PP2B) can form a tertiary complex, suggesting that both PKA and calcineurin are targeted by a common protein to subcellular sites where they regulate the phosphorylation status of key substrates.

610314 Rev. 1
格式详情
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610314 Rev.1
报价单和参考
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研发参考 (4)

  1. Carr DW, Stofko-Hahn RE, Fraser ID, Cone RD, Scott JD. Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. Characterization of AKAP 79.. J Biol Chem. 1992; 267(24):16816-23. (Biology). 查看参考
  2. Coghlan VM, Perrino BA, Howard M. Association of protein kinase A and protein phosphatase 2B with a common anchoring protein. Science. 1995; 267(5194):108-111. (Biology). 查看参考
  3. Jicha GA, Weaver C, Lane E. cAMP-dependent protein kinase phosphorylations on tau in Alzheimer's disease. J Neurosci. 1999; 19(17):7486-7494. (Clone-specific: Immunohistochemistry, Western blot). 查看参考
  4. Schillace RV, Andrews SF, Liberty GA, Davey MP, Carr DW. Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes. J Immunol. 2002; 168(4):1590-1599. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). 查看参考
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610314 Rev. 1

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